German-Retana S, Bergey B, Delbos R P, Candresse T, Dunez J
Station de Pathologie Vegétale, INRA Villenave d'Ornon, France.
Arch Virol. 1997;142(4):833-41. doi: 10.1007/s007050050122.
The genome of the Balaton 1 severe cherry isolate of apple chlorotic leaf spot trichovirus (ACLSV-Bal1) has been cloned and sequenced. The genomic RNA is 7549 nucleotide long, excluding the poly A tail. The genomic organization, with three overlapping open reading frames (ORF), is similar to that of the other sequenced ACLSV isolates. Sequence comparisons indicate a high variability between ACLSV isolates, with overall nucleotide sequence homology levels between 76 and 82%. The coat protein, encoded internally inside a larger ORF, is the most conserved protein (identity levels between 87 and 93%) while the central ORF, encoding the putative movement protein, is the most divergent (77 to 85% identity).
苹果褪绿叶斑毛形病毒（ACLSV）巴拉顿1号严重樱桃分离株（ACLSV-Bal1）的基因组已被克隆并测序。基因组RNA长7549个核苷酸，不包括聚腺苷酸尾。其基因组结构有三个重叠的开放阅读框（ORF），与其他已测序的ACLSV分离株相似。序列比较表明ACLSV分离株之间存在高度变异性，总体核苷酸序列同源性水平在76%至82%之间。外壳蛋白在一个较大的ORF内部编码，是最保守的蛋白（同一性水平在87%至93%之间），而编码假定移动蛋白的中央ORF是差异最大的（同一性为77%至85%）。
