Teerlink T, Hennekes M W, Mulder C, Brulez H F
Department of Clinical Chemistry, Free University Hospital, Amsterdam, Netherlands.
J Chromatogr B Biomed Sci Appl. 1997 Apr 11;691(2):269-76. doi: 10.1016/s0378-4347(96)00476-8.
A reversed-phase HPLC method for the quantification of dimethylamine in serum and urine is presented. Dimethylamine (DMA) is converted into a stable fluorescent product by precolumn derivatization with fluorenylmethylchloroformate. The DMA derivative is resolved from derivatives of other amines and amino acids by gradient elution with a total run-time of 15 min. The lower limit of determination in biological samples is 0.1 micromol/l. Recoveries from spiked serum samples were 99-107%. Within- and between-run precision were better than 6%. Concentrations of DMA in serum from normal human subjects (n=8) and from continuous ambulatory peritoneal dialysis patients (n=15) were 3.3+/-1.5 and 29.1+/-12.1 micromol/l, respectively.
本文介绍了一种用于定量血清和尿液中二甲胺的反相高效液相色谱法。二甲胺（DMA）通过用芴甲氧羰基氯进行柱前衍生化转化为稳定的荧光产物。通过梯度洗脱将DMA衍生物与其他胺类和氨基酸的衍生物分离，总运行时间为15分钟。生物样品中的测定下限为0.1微摩尔/升。加标血清样品的回收率为99 - 107%。批内和批间精密度均优于6%。正常人类受试者（n = 8）和持续性非卧床腹膜透析患者（n = 15）血清中DMA的浓度分别为3.3±1.5和29.1±12.1微摩尔/升。
