神经生长因子（NGF）和脑源性神经营养因子（BDNF）可增强来自胚胎大鼠中隔的培养神经元中囊泡乙酰胆碱转运体的免疫反应性。
NGF and BDNF increase the immunoreactivity of vesicular acetylcholine transporter in cultured neurons from the embryonic rat septum.
作者信息
Takei N, Kuramoto H, Endo Y, Hatanaka H
机构信息
Department of Applied Biology, Kyoto Institute of Technology, Sakyo, Japan.
出版信息
Neurosci Lett. 1997 May 2;226(3):207-9. doi: 10.1016/s0304-3940(97)00284-x.
The expression of vesicular acetylcholine transporter (VAChT), which transports ACh into synaptic vesicles, is coregulated with choline acetyltransferase (ChAT). Therefore, the effects of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) on the levels of VAChT in cultured neurons from the septum of embryonic rats were investigated by immunocytochemistry. NGF and BDNF increased the number of VAChT-immunoreactive neurons by approximately 1.5-fold and enhanced the immunoreactivity in each positive cell. These results suggest that the neurotrophins enhance not only synthesis but also storage of ACh in septal neurons.
将乙酰胆碱转运至突触小泡的囊泡乙酰胆碱转运体（VAChT）的表达与胆碱乙酰转移酶（ChAT）共同调节。因此，通过免疫细胞化学方法研究了神经生长因子（NGF）和脑源性神经营养因子（BDNF）对胚胎大鼠隔区培养神经元中VAChT水平的影响。NGF和BDNF使VAChT免疫反应性神经元的数量增加了约1.5倍，并增强了每个阳性细胞中的免疫反应性。这些结果表明，神经营养因子不仅增强了隔区神经元中乙酰胆碱的合成，还增强了其储存。
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