Nĕmcová V, Petrovický P, ten Donkelaar H J
Anatomical Institute of the First Medical Faculty, Charles University, Prague, Czech Republic.
J Hirnforsch. 1997;38(2):231-41.
37 complete frontal and horizontal series of rat brain were studied to compare the distribution of choline acetyltransferase- (ChAT), tyrosine hydroxylase- (TH), substance P- (SP), calbindin D- (Calb) and NADPH-diaphorase (NADPH-d)-positive cells within the cytoarchitectonic borders of the latero-dorsal tegmental nucleus (L-D) and its neighbourhood. We found the same distribution, number and morphology of NADPH-d-positive cells and ChAT-positive cells. Rostrally, there are no borders between NADPH-d-positive cells of L-D and NADPH-d-positive cells of the lateral part of the dorsal raphe nucleus. Only a few TH-positive cells are intermingled with ChAT/NADPH-d-positive cells at the lateral border of L-D. TH-positive cells are larger or the same size as cholinergic neutrons. Locus coeruleus and its rostral part is full of TH-positive cells and their fibres run ventromedially towards L-D. Barrington's nucleus appears in double staining (ChAT and TH or NADPH-d and TH) as an empty area bordered by ChAT- or NADPH-d-positive cells of L-D and TH-positive fibres of the locus coeruleus. Some of these fibres run through the Barrington's nucleus. The shape and size of SP-positive neurons is the same as ChAT- and NADPH-d-positive neurons. SP-positive neurons are sparsely distributed in all parts of L-D, but there are only a few SP-positive cells in its medial part. About 50% of the ChAT- and NADPH-d-positive cells are also SP-positive. Results are expressed by figures in three representative frontal sections and one horizontal section through the dorsal mesopontine tegmentum.
研究了37组完整的大鼠脑额叶和水平系列切片，以比较胆碱乙酰转移酶（ChAT）、酪氨酸羟化酶（TH）、P物质（SP）、钙结合蛋白D（Calb）和NADPH黄递酶（NADPH-d）阳性细胞在背外侧被盖核（L-D）及其邻域的细胞构筑边界内的分布。我们发现NADPH-d阳性细胞和ChAT阳性细胞的分布、数量和形态相同。在嘴侧，L-D的NADPH-d阳性细胞与中缝背核外侧部分的NADPH-d阳性细胞之间没有边界。在L-D的外侧边界，只有少数TH阳性细胞与ChAT/NADPH-d阳性细胞混合。TH阳性细胞比胆碱能神经元大或大小相同。蓝斑及其嘴侧部分充满TH阳性细胞，其纤维向腹内侧延伸至L-D。在双重染色（ChAT和TH或NADPH-d和TH）中，巴林顿核表现为一个空白区域，其边界为L-D的ChAT或NADPH-d阳性细胞以及蓝斑的TH阳性纤维。其中一些纤维穿过巴林顿核。SP阳性神经元的形状和大小与ChAT和NADPH-d阳性神经元相同。SP阳性神经元稀疏分布于L-D的所有部位，但其内侧部分只有少数SP阳性细胞。约50%的ChAT和NADPH-d阳性细胞也是SP阳性。结果通过三张代表性额叶切片和一张穿过脑桥背侧中脑被盖的水平切片中的图表示。
