Simpson N A, Nimrod C, De Vermette R, Fournier J
Division of Perinatology, Ottawa General Hospital, Ontario, Canada.
Placenta. 1997 May;18(4):287-93. doi: 10.1016/s0143-4004(97)80063-x.
The process of placentation in the macaque has been extensively studied and found to resemble closely that observed in the human. In this model, histopathologically, intervillous flow is anticipated from week 3 post-conception. We set out to document the nature and onset of intervillous flow in the macaque in vivo using colour Doppler imaging (CDI), colour Doppler energy (CDE) and pulsed-wave Doppler (PWD). Pregnant females were assessed between 15-50 days gestation (term = 165 days) with an Acuson 128/XP10 high-resolution ultrasound scanner, using a 7-MHz linear array probe. The placenta, subjacent decidua and myometrium were assessed using CDI and CDE. Specific regions of flow were interrogated using PWD; the resulting flow velocity waveforms were stored and quantified using conventional Doppler indices. B-mode sonography was able to demonstrate the well-defined placental-decidual interface observed in this species; CDI and CDE clearly visualized the uteroplacental vasculature. Spiral arteries were followed to their point of discharge into the intervillous space, and PWD at these sites obtained a characteristic flow velocity waveform. The indices obtained confirmed a flow of low resistance and pulsatility throughout the gestation studied. Flow within the intervillous space was noted from day 20 of gestation.
猕猴胎盘形成的过程已得到广泛研究，发现与人类观察到的过程极为相似。在这个模型中，从组织病理学角度来看，预计在受孕后第3周会出现绒毛间隙血流。我们着手使用彩色多普勒成像（CDI）、彩色多普勒能量图（CDE）和脉冲波多普勒（PWD）来记录猕猴体内绒毛间隙血流的性质和起始情况。使用Acuson 128/XP10高分辨率超声扫描仪，配备7兆赫线性阵列探头，对妊娠15至50天（孕期为165天）的怀孕雌性猕猴进行评估。使用CDI和CDE对胎盘、其下方的蜕膜和子宫肌层进行评估。使用PWD对特定血流区域进行检测；将得到的血流速度波形存储起来，并使用传统多普勒指标进行量化。B型超声能够显示出在该物种中观察到的界限清晰的胎盘 - 蜕膜界面；CDI和CDE清晰地显示出子宫胎盘血管系统。追踪螺旋动脉至其排入绒毛间隙的部位，在这些部位使用PWD获得特征性的血流速度波形。所获得的指标证实，在整个研究的妊娠期内，血流具有低阻力和低搏动性的特点。在妊娠第20天就注意到了绒毛间隙内的血流。
