Lindh M, Andersson A S, Gusdal A
Department of Clinical Virology, Göteborg University, Sweden.
J Infect Dis. 1997 Jun;175(6):1285-93. doi: 10.1086/516458.
The nucleotide at position 1858 of hepatitis B virus has importance in chronic hepatitis B (HB) because a cytosine at nt 1858 effectively prevents virus escape through the precore TAG stop codon mutation. The relatedness between nt 1858 and genotypes was analyzed using a new genotyping method based on restriction fragment length polymorphism (RFLP) analysis of an S gene amplicon. Seventy-three gene bank sequences were analyzed by phylogenetic tree construction and RFLP prediction. A tree supporting the existence of 6 genotypes (A-F) was obtained, with C-1858 found in 9 of 9 A genotypes, 0 of 7 B, 0 of 19 C, 1 of 10 D, 0 of 2 E, and 3 of 3 F. Serum samples from 187 HB e antigen-positive chronic carriers were analyzed: Genotypes in northern Europeans were 60% A, 31% D; in southern Europeans and Middle Easterners 96% D; in Africans 53% A, 27% D, 20% E; and in East Asians 14% A, 43% B, 43% C. Cytosine at nt 1858 was found in 36 of 44 A, 0 of 32 B, 8 of 34 C, 0 of 59 D, 0 of 3 E, and 1 of 1 F genotype samples.
乙肝病毒1858位核苷酸在慢性乙肝中具有重要意义，因为1858位核苷酸处的胞嘧啶可有效阻止病毒通过前核心TAG终止密码子突变实现逃逸。基于S基因扩增子的限制性片段长度多态性（RFLP）分析的新基因分型方法，对1858位核苷酸与基因型之间的相关性进行了分析。通过系统发育树构建和RFLP预测对73个基因库序列进行了分析。得到了一棵支持6种基因型（A - F）存在的树，在9种A基因型中的9个、7种B基因型中的0个、19种C基因型中的0个、10种D基因型中的1个、2种E基因型中的0个以及3种F基因型中的3个中发现了C - 1858。对187例乙肝e抗原阳性慢性携带者的血清样本进行了分析：北欧人的基因型为60% A、31% D；南欧人和中东人为96% D；非洲人为53% A、27% D、20% E；东亚人为14% A、43% B、43% C。在44种A基因型样本中的36个、32种B基因型样本中的0个、34种C基因型样本中的8个、59种D基因型样本中的0个、3种E基因型样本中的0个以及1种F基因型样本中的1个中发现了1858位核苷酸处的胞嘧啶。
