Hachitanda Y, Saito M, Mori T, Hamazaki M
Department of Pathology, National Children's Hospital, Tokyo, Japan.
Med Pediatr Oncol. 1997 Aug;29(2):135-8. doi: 10.1002/(sici)1096-911x(199708)29:2<135::aid-mpo12>3.0.co;2-e.
Fluorescence in situ hybridization (FISH) was applied to neuroblastoma for detection of N-myc (MYCN) oncogene amplification, and the results were compared with Southern blot analysis (Southern). In nine neuroblastomas (formalin-fixed paraffin-embedded tissues were available in seven cases including two cases with touch preparations, and two cell lines), all five cases with N-myc amplification detected by Southern had cells with multiple N-myc signals by FISH, and three cases showed no N-myc amplification either by Southern or FISH procedure. One case, not examined by Southern, showed amplified signals of N-myc by FISH. These data indicate that FISH results for N-myc amplification have close correlation with Southern blot analysis. The chromosome 2-specific repetitive DNA probe was also applied for the analysis of ploidy by FISH. Six cases with N-myc amplification by Southern and/or FISH had diploid tumors and two cases without amplified N-myc showed aneuploidy. The remaining one case consisted of heterogeneous elements showing diploidy in undifferentiated tissue and both aneuploidy (ganglionic cells) and diploidy (Schwann cells) in differentiated area. We conclude that FISH is a practical, useful and reliable method over Southern especially for analysis of N-myc amplification in neuroblastoma, and simultaneous cohybridization with a specific chromosome probe is of great value in predicting the prognosis of patients.
荧光原位杂交（FISH）技术被应用于神经母细胞瘤，以检测N - myc（MYCN）癌基因扩增，并将结果与Southern印迹分析（Southern法）进行比较。在9例神经母细胞瘤中（7例有福尔马林固定石蜡包埋组织，包括2例有触摸涂片，2株细胞系），Southern法检测出的5例N - myc扩增病例，FISH检测均显示细胞有多个N - myc信号，3例Southern法和FISH法均未显示N - myc扩增。1例未进行Southern法检测的病例，FISH检测显示N - myc信号扩增。这些数据表明，FISH检测N - myc扩增的结果与Southern印迹分析密切相关。染色体2特异性重复DNA探针也被用于通过FISH分析倍性。Southern法和/或FISH法检测出的6例N - myc扩增病例肿瘤为二倍体，2例未扩增N - myc的病例显示为非整倍体。其余1例由异质性成分组成，未分化组织显示为二倍体，分化区域既有非整倍体（神经节细胞）又有二倍体（施万细胞）。我们得出结论，FISH是一种比Southern法更实用、有用且可靠的方法，尤其适用于分析神经母细胞瘤中的N - myc扩增，与特定染色体探针同时共杂交在预测患者预后方面具有重要价值。
