Vidal D R, Ragot C, Thibault F
Unité de Microbiologie, Centre de Recherches du Service de Santé des Armées, La Tronche.
Ann Pharm Fr. 1997;55(2):49-54.
When bacteria grow in close association with solid surface, they constitute a microbial community tight included in the exopolymer glycocalyx. Many laboratory studies have shown that these bacteria are 10 to 100 folds more resistant to disinfectants than the bacteria of the same strain in suspension. Several factors are responsible for this resistance: the glycocalyx which limits the diffusion and reacts with the disinfectant, the more or less dense repartition of the bacteria inside the biofilm, their physiologic state with reduced metabolism, and the surface on which is the biofilm. The activity assessment of disinfectant agents is achieved with standardized methods. They must take into account not only the conditions in which the disinfectants are employed, but also the micro-organism state. Experimental results showing the resistance of biofilm bacteria must lead to elaborate methods allowing the assessment of bactericide activity of disinfectants against biofilm bacteria.
当细菌在与固体表面紧密结合的情况下生长时，它们会构成一个紧密包含在胞外聚合物糖萼中的微生物群落。许多实验室研究表明，这些细菌对消毒剂的抵抗力比同一菌株在悬浮状态下的细菌高10至100倍。造成这种抵抗力的因素有几个：限制扩散并与消毒剂发生反应的糖萼、生物膜内细菌分布的或多或少的密度、代谢降低的生理状态以及生物膜所在的表面。消毒剂的活性评估是通过标准化方法进行的。这些方法不仅要考虑使用消毒剂的条件，还要考虑微生物的状态。显示生物膜细菌抵抗力的实验结果必须促使人们制定出能够评估消毒剂对生物膜细菌杀菌活性的方法。
