Beauvais-Jouneau A, Delouvée A, Craig S E, Humphries M J, Thiery J P, Dufour S
Subcellular Structure and Cellular Dynamics, UMR 144, CNRS-Institut Curie, Paris, France.
Exp Cell Res. 1997 May 25;233(1):1-10. doi: 10.1006/excr.1997.3554.
We have analyzed the interaction of neural crest cells with fragments of fibronectin corresponding to the different spliced variants of the COOH-terminal cell-binding domain (COOH-ter CBD). We have shown that this domain can support cell adhesion and migration and that both the IIICS and HepII regions are involved in these events. The rate of locomotion is high, although undirectional, compared to that of whole fibronectin. Interactions with the COOH-ter CBD are controlled by alpha4beta1 and maybe other beta1 integrins and cell-surface proteoglycans. These receptors act cooperatively to mediate attachment, spreading, and migration on fibronectin.
我们分析了神经嵴细胞与纤连蛋白片段的相互作用，这些片段对应于COOH末端细胞结合结构域（COOH-ter CBD）的不同剪接变体。我们已经表明，该结构域可以支持细胞粘附和迁移，并且IIICS和HepII区域都参与了这些过程。与完整的纤连蛋白相比，尽管运动无方向性，但运动速率较高。与COOH-ter CBD的相互作用由α4β1以及可能的其他β1整合素和细胞表面蛋白聚糖控制。这些受体协同作用，介导在纤连蛋白上的附着、铺展和迁移。
