抗重组40-kDa外膜蛋白单克隆抗体对牙龈卟啉单胞菌与粘性放线菌ATCC 19246之间定植因子的抑制作用
Inhibition of a Porphyromonas gingivalis colonizing factor between Actinomyces viscosus ATCC 19246 by monoclonal antibodies against recombinant 40-kDa outer-membrane protein.
作者信息
Saito S, Hiratsuka K, Hayakawa M, Takiguchi H, Abiko Y
机构信息
Department of Biochemistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
出版信息
Gen Pharmacol. 1997 May;28(5):675-80. doi: 10.1016/s0306-3623(96)00366-7.
PMID:9184801
Abstract
- Porphyromonas gingivalis, an important pathogen in human periodontal disease, aggregates with Actinomyces viscosus ATCC 19246. 2. Monoclonal antibodies (mAbs) against purified recombinant 40-kDa outer-membrane protein (r40-kDa, OMP) of P. gingivalis 381 inhibited its coaggregation with A. viscosus ATCC 19246 in a dose-dependent manner. 3. Five mAb clones against r40-kDa OMP were selected. The isotype of the five was IgG1. 4. Pg-ompA2 inhibited the coaggregation of several strains of P. gingivalis with A. viscosus ATCC 19246 cells.
摘要
- 牙龈卟啉单胞菌是人类牙周疾病中的一种重要病原体，它与粘性放线菌ATCC 19246聚集。2. 针对牙龈卟啉单胞菌381纯化重组40 kDa外膜蛋白（r40-kDa，OMP）的单克隆抗体（mAb）以剂量依赖方式抑制其与粘性放线菌ATCC 19246的共聚集。3. 选择了5个针对r40-kDa OMP的单克隆抗体克隆。这5个的同种型为IgG1。4. Pg-ompA2抑制了几株牙龈卟啉单胞菌与粘性放线菌ATCC 19246细胞的共聚集。
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