用酶联免疫吸附试验检测人体感染巨片形吸虫时，成虫体抗原与排泄-分泌抗原的比较用于血清学诊断。
Comparison of adult somatic and excretory-secretory antigens in enzyme-linked immunosorbent assay for serodiagnosis of human infection with Fasciola gigantica.
作者信息
Maleewong W, Intapan P M, Wongkham C, Tomanakan K, Daenseekaew W, Sukeepaisarnjaroen W
机构信息
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Thailand.
出版信息
Southeast Asian J Trop Med Public Health. 1996 Sep;27(3):566-9.
Adult somatic antigen extract of Fasciola gigantica was compared with excretory-secretory (ES) antigen in an enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of human fascioliasis gigantica. The absorbance values in ELISA using the adult somatic antigen were not significantly different from the values obtaining using ES antigen (p > 0.05). The diagnostic sensitivity, specificity and positive and negative predictive values of the test using adult somatic extract as antigen were 100%, 98%, 70% and 100%, respectively. On the other hand, these values of the test using adult ES antigen were 100%, 99.3%, 87.5% and 100%, respectively. It appears that both somatic and ES antigens are effective antigens for use in the serodiagnosis of human fascioliasis gigantica.
在酶联免疫吸附测定（ELISA）中，对巨大片形吸虫的成虫体抗原提取物与排泄-分泌（ES）抗原进行了比较，用于人体巨大片形吸虫病的血清学诊断。使用成虫体抗原的ELISA吸光度值与使用ES抗原获得的值无显著差异（p>0.05）。以成虫体提取物为抗原的检测的诊断敏感性、特异性、阳性和阴性预测值分别为100%、98%、70%和100%。另一方面，使用成虫ES抗原的检测的这些值分别为100%、99.3%、87.5%和100%。看来体抗原和ES抗原都是用于人体巨大片形吸虫病血清学诊断的有效抗原。
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