Tay S T, Nazma S, Rohani M Y
Bacteriology Division, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia.
Southeast Asian J Trop Med Public Health. 1996 Sep;27(3):580-3.
A rapid diagnostic system for scrub typhus using nested polymerase chain reaction (PCR) was applied to clinical samples from Malaysian Aborigines. Whole blood from twenty-four patients suspected of scrub typhus infection were tested using nested polymerase chain reaction and sera were evaluated by the indirect immunoperoxidase test. Antibody responses towards Rickettsia tsutsugamushi were observed in seventeen patients with the majority having high titers of IgG antibodies. Seven patients were seronegative. The nested PCR amplified R. tsutsugamushi DNA from six patients, of which two were negative serologically and four had high titers of IgG antibodies. Second samples collected seven days after treatment were negative by PCR testing. Nested PCR is highly sensitive and specific and may be used to provide rapid confirmation of scrub typhus cases in endemic region.
一种使用巢式聚合酶链反应（PCR）的恙虫病快速诊断系统应用于马来西亚原住民的临床样本。对24名疑似恙虫病感染患者的全血进行巢式聚合酶链反应检测，并通过间接免疫过氧化物酶试验评估血清。在17名患者中观察到针对恙虫病立克次体的抗体反应，大多数患者具有高滴度的IgG抗体。7名患者血清学阴性。巢式PCR从6名患者中扩增出恙虫病立克次体DNA，其中2名血清学阴性，4名具有高滴度的IgG抗体。治疗7天后采集的第二份样本经PCR检测为阴性。巢式PCR高度敏感且特异，可用于在流行地区快速确诊恙虫病病例。
