DDRT-PCR 产物再扩增及克隆的优化 - Suppr

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Refinements in re-amplification and cloning of DDRT-PCR products.

作者信息

Bhattacharjee A, Rutherford M S, Abrahamsen M S, Lappi V R, Schook L B

机构信息

University of Minnesota, St. Paul, USA.

出版信息

Biotechniques. 1997 Jun;22(6):1048-51. doi: 10.2144/97226bm08.

DOI:10.2144/97226bm08

PMID:9187749

Abstract

摘要

相似文献

Refinements in re-amplification and cloning of DDRT-PCR products.

Biotechniques. 1997 Jun;22(6):1048-51. doi: 10.2144/97226bm08.

PCR strategy to obtain the 5' and 3' flanking sequences of partial clones from lambda ZAP cDNA libraries.

Biotechniques. 1997 Jun;22(6):1038-40, 1042, 1044. doi: 10.2144/97226bm06.

Identification and cloning of differentially expressed genes by DDRT-PCR.

Methods Mol Biol. 1997;67:419-30. doi: 10.1385/0-89603-483-6:419.

pSDTV vector: a modification of the pBluescript SK+ plasmid in order to perform PCR-fragments TA-cloning using Eam1105I restriction endonuclease.

Mol Biol Rep. 2012 May;39(5):6133-9. doi: 10.1007/s11033-011-1429-3. Epub 2011 Dec 28.

Agarose gel size fractionation of RNA for the cloning of full-length cDNAs.

Anal Biochem. 2003 Dec 15;323(2):252-5. doi: 10.1016/j.ab.2003.10.007.

Amplification of cDNA ends using PCR suppression effect and step-out PCR.

Methods Mol Biol. 2003;221:41-9. doi: 10.1385/1-59259-359-3:41.

PCR amplification of cDNA libraries for cloning and screening.

Biotechniques. 1998 Apr;24(4):574-5. doi: 10.2144/98244bm11.

Differential display. A general protocol.

Methods Mol Biol. 1997;85:3-11. doi: 10.1385/0-89603-489-5:3.

Description of the entire mRNA population by a 3' end cDNA fragment generated by class IIS restriction enzymes.

Nucleic Acids Res. 1995 Sep 25;23(18):3685-90. doi: 10.1093/nar/23.18.3685.

[A novel T-cloning vector system].

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引用本文的文献

Prevention of depurination during elution facilitates the reamplification of DNA from differential display gels.

Nucleic Acids Res. 1999 Aug 1;27(15):e6. doi: 10.1093/nar/27.15.e6.

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Refinements in re-amplification and cloning of DDRT-PCR products.

作者信息

Bhattacharjee A, Rutherford M S, Abrahamsen M S, Lappi V R, Schook L B

机构信息

University of Minnesota, St. Paul, USA.

出版信息

Biotechniques. 1997 Jun;22(6):1048-51. doi: 10.2144/97226bm08.

DOI:10.2144/97226bm08

PMID:9187749

Abstract

摘要

相似文献

Refinements in re-amplification and cloning of DDRT-PCR products.

Biotechniques. 1997 Jun;22(6):1048-51. doi: 10.2144/97226bm08.

PCR strategy to obtain the 5' and 3' flanking sequences of partial clones from lambda ZAP cDNA libraries.

Biotechniques. 1997 Jun;22(6):1038-40, 1042, 1044. doi: 10.2144/97226bm06.

Identification and cloning of differentially expressed genes by DDRT-PCR.

Methods Mol Biol. 1997;67:419-30. doi: 10.1385/0-89603-483-6:419.

pSDTV vector: a modification of the pBluescript SK+ plasmid in order to perform PCR-fragments TA-cloning using Eam1105I restriction endonuclease.

Mol Biol Rep. 2012 May;39(5):6133-9. doi: 10.1007/s11033-011-1429-3. Epub 2011 Dec 28.

Agarose gel size fractionation of RNA for the cloning of full-length cDNAs.

Anal Biochem. 2003 Dec 15;323(2):252-5. doi: 10.1016/j.ab.2003.10.007.

Amplification of cDNA ends using PCR suppression effect and step-out PCR.

Methods Mol Biol. 2003;221:41-9. doi: 10.1385/1-59259-359-3:41.

PCR amplification of cDNA libraries for cloning and screening.

Biotechniques. 1998 Apr;24(4):574-5. doi: 10.2144/98244bm11.

Differential display. A general protocol.

Methods Mol Biol. 1997;85:3-11. doi: 10.1385/0-89603-489-5:3.

Description of the entire mRNA population by a 3' end cDNA fragment generated by class IIS restriction enzymes.

Nucleic Acids Res. 1995 Sep 25;23(18):3685-90. doi: 10.1093/nar/23.18.3685.

[A novel T-cloning vector system].

Mikrobiyol Bul. 2004 Jul;38(3):239-43.

引用本文的文献

Prevention of depurination during elution facilitates the reamplification of DNA from differential display gels.

Nucleic Acids Res. 1999 Aug 1;27(15):e6. doi: 10.1093/nar/27.15.e6.