Kolberg J, Høiby E A, Jantzen E
Department of Vaccinology, National Institute of Public Health, Oslo, Norway.
Microb Pathog. 1997 Jun;22(6):321-9. doi: 10.1006/mpat.1996.0114.
The phosphorylcholine (PC) determinant in Streptococcus pneumoniae is known to be linked to the cell wall polysaccharides (C-Ps) and to the lipoteichoic acid (LTA) (Forssman antigen) of the plasma membrane. Western blotting with two PC specific murine monoclonal antibodies (MAbs) designated 145,F-2 (IgM) and 147,A-1 (IgA) showed a similar ladder-like pattern for all examined strains of S. pneumoniae and Streptococcus mitis. Purified antigens run in parallel indicated that this ladder pattern is due to the PC of LTA. Unlike other techniques, Western blotting thus enables the identification of only one of the streptococcal structures carrying the PC epitope. Gram-negative organisms were also examined, and six of 11 Haemophilus influenzae strains reacted with the MAbs. For this species, unlike the streptococci, only one fast moving band was detected. Analyses by thin-layer chromatography (TLC) detected the PC epitope in lipopolysaccharide (LPS) fraction from H. influenzae. Some strains of the Neisseriaceae family were also positive by Western blotting, but TLC and immunostaining did not detect the PC determinant in LPS.
已知肺炎链球菌中的磷酰胆碱（PC）决定簇与细胞壁多糖（C-Ps）以及质膜的脂磷壁酸（LTA）（福斯曼抗原）相连。用两种PC特异性鼠单克隆抗体（MAb）进行的蛋白质印迹分析，这两种抗体分别命名为145,F-2（IgM）和147,A-1（IgA），结果显示，所有检测的肺炎链球菌和缓症链球菌菌株都呈现出相似的梯状模式。并行运行的纯化抗原表明，这种梯状模式是由LTA的PC引起的。与其他技术不同，蛋白质印迹分析因此只能鉴定携带PC表位的一种链球菌结构。还对革兰氏阴性菌进行了检测，11株流感嗜血杆菌中有6株与这些单克隆抗体发生反应。对于该物种，与链球菌不同，只检测到一条快速移动的条带。通过薄层色谱（TLC）分析在流感嗜血杆菌的脂多糖（LPS）组分中检测到了PC表位。奈瑟菌科的一些菌株通过蛋白质印迹分析也呈阳性，但TLC和免疫染色未在LPS中检测到PC决定簇。
