Rahman M, Jonsson A B, Holme T
Microbiology and Tumorbiology Center, Karolinska Institute, Stockholm, S-171 77, Sweden.
Microb Pathog. 1998 May;24(5):299-308. doi: 10.1006/mpat.1997.0191.
Murine monoclonal antibodies (MAbs) against the A, B and C LPS serotypes of M. catarrhalis were generated and their binding specificity was examined in an enzyme-linked immunosorbent assay (ELISA). Two broadly cross-reactive monoclonal antibodies (MCA1 and MCC2) against the outer core region of LPS were further characterized. A panel of synthetic glycoproteins and glycolipids was used to determine the binding specificity of the MAbs. MCA1 and MCC2 bound specifically to alpha-Gal-(1-4)-beta-Gal of galabiose and globotriose glycoconjugates. The reactivity of the MAbs with galabiose was higher than that with globotriose. The MAbs could recognize the alpha-Gal-(1-4)-beta-Gal epitope only when it was in a terminal position. MCA1 was further shown to react with a similar epitope in the glycosylated type IV pili of N. meningitidis, which has been shown to contain a 1-4 linked digalactose at the terminal part of the saccharide present in the pili. MCA1 could efficiently recognize this epitope indicating that it was exposed on the surface of the pili.
制备了针对卡他莫拉菌A、B和C脂多糖血清型的鼠单克隆抗体（MAb），并在酶联免疫吸附测定（ELISA）中检测了它们的结合特异性。进一步对两种针对脂多糖外核心区域的广泛交叉反应性单克隆抗体（MCA1和MCC2）进行了表征。使用一组合成糖蛋白和糖脂来确定单克隆抗体的结合特异性。MCA1和MCC2特异性结合半乳糖和球三糖糖缀合物的α-Gal-(1-4)-β-Gal。单克隆抗体与半乳糖的反应性高于与球三糖的反应性。单克隆抗体仅在α-Gal-(1-4)-β-Gal表位处于末端位置时才能识别它。进一步证明MCA1与脑膜炎奈瑟菌糖基化IV型菌毛中的类似表位发生反应，该菌毛已显示在菌毛中存在的糖的末端部分含有1-4连接的二半乳糖。MCA1能够有效识别该表位，表明它暴露在菌毛表面。
