Ritsema T, Lugtenberg B J, Spaink H P
Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University, The Netherlands.
J Bacteriol. 1997 Jun;179(12):4053-5. doi: 10.1128/jb.179.12.4053-4055.1997.
NodA controls transfer of a fatty acid in the biosynthesis of lipochitin oligosaccharides by rhizobia. In an in vitro assay, we used de-N-acetylated chitin oligosaccharides substituted with an O-acetyl moiety as acyl acceptor substrates. We show that acyl-acyl carrier protein is used as a donor in NodA-directed fatty acid transfer.
NodA在根瘤菌脂壳寡糖生物合成过程中控制脂肪酸的转移。在体外试验中,我们使用了用O - 乙酰基部分取代的脱N - 乙酰化壳寡糖作为酰基受体底物。我们发现酰基 - 酰基载体蛋白在NodA介导的脂肪酸转移中用作供体。