马铃薯卷叶病毒颗粒中马铃薯纺锤块茎类病毒异源衣壳化的证据。

Evidence for heterologous encapsidation of potato spindle tuber viroid in particles of potato leafroll virus.

作者信息

Querci M, Owens R A, Bartolini I, Lazarte V, Salazar L F

机构信息

Molecular Virology Laboratory, International Potato Center, Lima, Peru.

出版信息

J Gen Virol. 1997 Jun;78 ( Pt 6):1207-11. doi: 10.1099/0022-1317-78-6-1207.

DOI:10.1099/0022-1317-78-6-1207

PMID:9191909

Abstract

The aphid Myzus persicae (Sulz.) was shown to transmit potato spindle tuber viroid (PSTVd) to potato clone DTO-33 from source plants doubly infected with potato leafroll virus (PLRV) and PSTVd. Transmission was of the persistent type and did not occur when the insects were allowed to feed on singly infected plants. Only low levels of PSTVd were associated with purified PLRV virions, but its resistance to digestion with micrococcal nuclease indicates that the viroid RNA is encapsidated within the PLRV particles. Epidemiological surveys carried out at three locations in China revealed a strong correlation between PSTVd infection and the presence of PLRV, suggesting that PLRV can facilitate PSTVd spread under field conditions.

摘要

已证明蚜虫桃蚜（Myzus persicae (Sulz.)）能将马铃薯纺锤块茎类病毒（PSTVd）从同时感染马铃薯卷叶病毒（PLRV）和PSTVd的源植物传播到马铃薯无性系DTO - 33。传播属于持久性类型，当让昆虫取食单一感染的植物时则不会发生传播。仅有低水平的PSTVd与纯化的PLRV病毒粒子相关联，但其对微球菌核酸酶消化的抗性表明类病毒RNA被包裹在PLRV粒子内。在中国三个地点进行的流行病学调查显示，PSTVd感染与PLRV的存在之间存在很强的相关性，这表明PLRV在田间条件下可促进PSTVd的传播。