表达双链RNA特异性核糖核酸酶的转基因马铃薯对马铃薯纺锤块茎类病毒具有抗性。

Transgenic potato expressing a double-stranded RNA-specific ribonuclease is resistant to potato spindle tuber viroid.

作者信息

Sano T, Nagayama A, Ogawa T, Ishida I, Okada Y

机构信息

Laboratory of Phytopathology, Faculty of Agriculture, Hirosaki University, Japan.

出版信息

Nat Biotechnol. 1997 Nov;15(12):1290-4. doi: 10.1038/nbt1197-1290.

DOI:10.1038/nbt1197-1290

PMID:9359114

Abstract

We have produced transgenic potato lines expressing the yeast-derived double-stranded RNA-specific ribonuclease pac1. Five lines of pac1 potato (Solanum tuberosum L., cultivar Russet Burbank) challenged with potato spindle tuber viroid (PSTVd) suppressed PSTVd infection and accumulation. All of the progeny potato tubers produced by resistant plants were also free of PSTVd. Because the pac1 gene product digested PSTVd in vitro, double-stranded regions in PSTVd molecule and/or replicative intermediates may be targeted by pac1 gene product in the transgenic potato plant.

摘要

我们已经培育出了表达酵母来源的双链RNA特异性核糖核酸酶pac1的转基因马铃薯品系。用马铃薯纺锤块茎类病毒（PSTVd）攻击五个pac1马铃薯品系（Solanum tuberosum L.，品种为褐皮伯班克），抑制了PSTVd的感染和积累。抗性植株产生的所有后代马铃薯块茎也都没有PSTVd。由于pac1基因产物在体外能消化PSTVd，因此PSTVd分子中的双链区域和/或复制中间体可能是转基因马铃薯植株中pac1基因产物的作用靶点。