Lupiani B, Reddy S M, Subramanian K, Samal S K
Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park 20742, USA.
J Gen Virol. 1997 Jun;78 ( Pt 6):1379-83. doi: 10.1099/0022-1317-78-6-1379.
The nucleotide and deduced amino acid sequences of genome segment 10 of aquareovirus strain SBR, encoding the major outer capsid protein (VP7), have been determined. Genome segment 10 of SBR virus is 986 nucleotides long and encodes a polypeptide of 298 amino acids with a predicted molecular mass of 32,430 Da. There are 26 non-translated nucleotides at the 5' end and 66 non-translated nucleotides at the 3' end. Using a recombinant baculovirus system, the VP7 protein of SBR virus was expressed to a high level. The baculovirus-produced VP7 protein was similar both in its size and antigenic properties to the authentic aquareovirus VP7 protein. Antiserum from a rabbit immunized with the baculovirus-produced VP7 protein failed to neutralize the homologous aquareovirus strain. As determined by Western blotting, this antiserum reacted with aquareovirus strains belonging to the same genogroup as SBR virus, but did not react with aquareovirus strains belonging to the other genogroups.
已确定水生呼肠孤病毒SBR株基因组第10节段的核苷酸序列及其推导的氨基酸序列，该节段编码主要外衣壳蛋白（VP7）。SBR病毒的基因组第10节段长986个核苷酸，编码一个由298个氨基酸组成的多肽，预测分子量为32,430道尔顿。5'端有26个非翻译核苷酸，3'端有66个非翻译核苷酸。利用重组杆状病毒系统，SBR病毒的VP7蛋白得以高水平表达。杆状病毒产生的VP7蛋白在大小和抗原特性上与天然水生呼肠孤病毒VP7蛋白相似。用杆状病毒产生的VP7蛋白免疫的兔抗血清不能中和同源水生呼肠孤病毒株。通过蛋白质免疫印迹法测定，该抗血清与属于与SBR病毒相同基因群的水生呼肠孤病毒株发生反应，但不与属于其他基因群的水生呼肠孤病毒株发生反应。
