Kusakari Y, Nishikawa S, Ishiguro S, Tamai M
Department of Ophthalmology, Tohoku University School of Medicine, Sendai, Japan.
Curr Eye Res. 1997 Jun;16(6):600-4. doi: 10.1076/ceyr.16.6.600.5084.
Histidine is an indispensable amino acid with an imidazole ring and it is a precursor of histamine, carnosine, and anserine. Histidine has been proposed to act as a neurotransmitter or neuromodulator in mammalian central nervous system (CNS), including retina. In this study, we report histidine-like immunoreactivity in the rat retina with the use of antibodies raised against histidine coupled to bovine serum albumin (BSA) with glutaraldehyde.
In order to confirm the specificity of the antiserum toward histidine, immunodots were carried out. Only the histidine conjugate showed immunoreactivity. The rat retinas fixed with glutaraldehyde were used for immunocytochemistry.
Histidine immunoreactivity was identified in the ganglion cell layer (GCL), inner nuclear layer (INL), inner plexiform layer (IPL), and Müller cells of rat retina.
Histidine may be a precursor of histamine in the inner retina, and Müller cells may play some role in the metabolism of histidine.
组氨酸是一种带有咪唑环的必需氨基酸，它是组胺、肌肽和鹅肌肽的前体。有人提出组氨酸在包括视网膜在内的哺乳动物中枢神经系统（CNS）中作为神经递质或神经调节剂发挥作用。在本研究中，我们报告了使用针对与牛血清白蛋白（BSA）偶联的组氨酸并经戊二醛处理后产生的抗体，在大鼠视网膜中检测到的组氨酸样免疫反应性。
为了确认抗血清对组氨酸的特异性，进行了免疫斑点试验。只有组氨酸缀合物显示出免疫反应性。用戊二醛固定的大鼠视网膜用于免疫细胞化学。
在大鼠视网膜的神经节细胞层（GCL）、内核层（INL）、内网状层（IPL）和Müller细胞中鉴定出组氨酸免疫反应性。
组氨酸可能是视网膜内层组胺的前体，并且Müller细胞可能在组氨酸的代谢中发挥某些作用。
