Stott I, Murthy A, Robinson A, Thomas N W, Fry J R
Department of Physiology and Pharmacology, Medical School, Queen's Medical Centre, Nottingham, UK.
Hum Exp Toxicol. 1997 May;16(5):262-6. doi: 10.1177/096032719701600505.
The effect of low doses of diethyldithiocarbamate (DEDC) on hepatic cytochrome P450-dependent enzyme activity and 1,3-dichloro-2-propanol (DCP) hepatotoxicity in the rat have been investigated. DEDC at a dose of 5 mg/kg selectively inhibited enzyme markers for CYP2E1 activity, and provided substantial protection against DCP hepatotoxicity. At a higher dose (25 mg/kg), DEDC also inhibited an enzyme marker for CYP1A2 activity and provided complete protection against DCP hepatotoxicity. It is concluded: (a) that DEDC at a dose of 5 mg/kg is a selective CYP2E1 inhibitor in the rat in vivo; and (b) that DCP hepatotoxicity is mediated principally by CYP2E1, with a possible contribution from CYP1A2.
研究了低剂量二乙氨基二硫代甲酸盐（DEDC）对大鼠肝脏细胞色素P450依赖性酶活性及1,3 - 二氯 - 2 - 丙醇（DCP）肝毒性的影响。5mg/kg剂量的DEDC选择性抑制CYP2E1活性的酶标志物，并对DCP肝毒性提供显著保护。在较高剂量（25mg/kg）时，DEDC还抑制CYP1A2活性的酶标志物，并对DCP肝毒性提供完全保护。得出以下结论：（a）5mg/kg剂量的DEDC在大鼠体内是一种选择性CYP2E1抑制剂；（b）DCP肝毒性主要由CYP2E1介导，CYP1A2可能也有一定作用。
