Matsukawa A, Fukumoto T, Maeda T, Ohkawara S, Yoshinaga M
Department of Pathology, Kumamoto University School of Medicine, Japan.
Cytokine. 1997 May;9(5):307-15. doi: 10.1006/cyto.1996.0170.
Interleukin 1 (IL-1) is postulated to function in maintaining homeostasis, however, over-action of this cytokine may lead to disruption of homeostasis due to it's wide spectrum of activities. To understand the endogenous regulation of this cytokine, we examined the existence of IL-1 receptor antagonist (IL-1Ra) in tissues from healthy rabbits. IL-1Ra was constitutively produced in all tissues examined (lung, liver, spleen, thymus, caecum, skin, kidney, heart, and brain), as estimated by ELISA. Immunoprecipitation, RT-PCR and immunohistochemical studies indicated that all tissues produced secreted form of IL-1Ra (sIL-1Ra), whereas thymus, caecum, skin and kidney produced both sIL-1Ra and intracellular of IL-1Ra. All tissue IL-1Ra purified using anti-IL-1Ra IgG affinity chromatography had inhibitory activity on the IL-1-induced thymocyte proliferative response, and the activity was totally abolished by anti-IL-1Ra mAb. No IL-1 activity was detected in any tissues except skin and heart, however, after preincubation of the samples with anti-IL-1Ra, the activity was first visible in the tissues. Under these conditions, IL-1 activity in skin and heart was enhanced to 170% and 280%, respectively. Taken together, we conclude that tissue IL-1Ra is involved in health maintenance by masking co-existing IL-1 activity present in tissues.
白细胞介素1（IL-1）被认为在维持体内平衡中发挥作用，然而，由于这种细胞因子具有广泛的活性，其过度作用可能导致体内平衡的破坏。为了了解这种细胞因子的内源性调节，我们检测了健康兔子组织中白细胞介素1受体拮抗剂（IL-1Ra）的存在情况。通过酶联免疫吸附测定（ELISA）估计，在所检测的所有组织（肺、肝、脾、胸腺、盲肠、皮肤、肾、心脏和脑）中均组成性产生IL-1Ra。免疫沉淀、逆转录聚合酶链反应（RT-PCR）和免疫组织化学研究表明，所有组织均产生分泌形式的IL-1Ra（sIL-1Ra），而胸腺、盲肠、皮肤和肾则同时产生sIL-1Ra和细胞内形式的IL-1Ra。使用抗IL-1Ra IgG亲和层析纯化的所有组织IL-1Ra对IL-1诱导的胸腺细胞增殖反应均具有抑制活性，且该活性被抗IL-1Ra单克隆抗体完全消除。除皮肤和心脏外，在任何组织中均未检测到IL-1活性，然而，在用抗IL-1Ra预孵育样品后，该活性首先在这些组织中显现出来。在这些条件下，皮肤和心脏中的IL-1活性分别增强至170%和280%。综上所述，我们得出结论，组织IL-1Ra通过掩盖组织中同时存在的IL-1活性参与健康维持。
