白细胞介素-2和白细胞介素-4对透析过的椎实螺神经元γ-氨基丁酸诱导的内向电流的相反作用。

Opposite effects of interleukin-2 and interleukin-4 on GABA-induced inward currents of dialysed Lymnaea neurons.

S-Rózsa K, Rubakhin S S, Szücs A, Hughes T K, Stefano G B

Balaton Limnological Research Institute, Hungarian Academy of Siences, Tihany, Hungary.

Gen Pharmacol. 1997 Jul;29(1):73-7. doi: 10.1016/s0306-3623(96)00527-7.

Abstract

The effects of interleukin-2 (rhIL-2) and interleukin-4 (rhIL-4) were investigated on gamma-aminobutyric acid (GABA)-induced inward currents on isolated, identified neurons of Lymnaea stagnalis L. (Mollusca, Gastropoda) by using a concentration clamp technique. 2. It was shown that the interleukins modified the GABA-induced inward current in an opposite direction: rhIL-2 (2-100 U/ml) decreased the peak value of IGABA in a dose-dependent manner, whereas rhIL-4 (0.2-100 U/ml), on the contrary, potentiated it. Both types of modulation were partially or fully reversible. 3. The reversal potential of IGABA was not shifted by these cytokines. 4. The time-to-peak value and inactivation time constant of the gamma-aminobutyric acid (GABA)-induced current was decreased by rhIL-4. The modulatory effect of rhIL-4 was eliminated after conjugation of this cytokine with its antibody. 5. It appears that cytokines could play a role in regulating the neural excitability through GABA-erg mechanisms.

摘要

采用浓度钳技术，研究了白细胞介素-2（rhIL-2）和白细胞介素-4（rhIL-4）对静水椎实螺（软体动物，腹足纲）分离的、已鉴定神经元上γ-氨基丁酸（GABA）诱导的内向电流的影响。2. 结果表明，白细胞介素以相反的方向改变GABA诱导的内向电流：rhIL-2（2-100 U/ml）以剂量依赖的方式降低IGABA的峰值，而rhIL-4（0.2-100 U/ml）则相反，使其增强。两种类型的调节都是部分或完全可逆的。3. 这些细胞因子并未改变IGABA的反转电位。4. rhIL-4降低了γ-氨基丁酸（GABA）诱导电流的峰值时间和失活时间常数。该细胞因子与其抗体结合后，rhIL-4的调节作用消失。5. 细胞因子似乎可以通过GABA能机制在调节神经兴奋性中发挥作用。