te Velde A A, Flendrig L M, Ladiges N C, Chamuleau R A
University of Amsterdam, Department of Experimental Internal Medicine, The Netherlands.
Int J Artif Organs. 1997 Apr;20(4):229-33.
The use of cells from xenogeneic origin in a bioartificial liver can have a number of immunological consequences, not only for the cells in the bioartificial liver but also for the patient receiving the bioartificial liver treatment. The impact of these consequences will depend on the immune status of the patient receiving bioartificial liver treatment, the duration and frequency of the treatment and on the extent of interaction between the patients blood (or plasma) and the xenogeneic liver cells. In an experimental model we infused rats with a culture supernatant of pig hepatocytes and demonstrated using Western blots and immunohistological techniques that antibodies are raised against the very small amounts of the pig hepatocyte-derived proteins present in the culture medium. Potential problems of bioartificial liver destruction and the possibility of hypersensitivity reactions due to the secretion of xenogeneic proteins into the circulation of the patient are discussed. Because the liver has an important role in the clearance of immune complexes it is concluded that precautions should be taken when (repeated) application of a xenogeneic bioartificial liver in patients with liver failure is considered.
在生物人工肝中使用来自异种来源的细胞可能会产生许多免疫学后果，这不仅会影响生物人工肝中的细胞，还会对接受生物人工肝治疗的患者产生影响。这些后果的影响程度将取决于接受生物人工肝治疗患者的免疫状态、治疗的持续时间和频率，以及患者血液（或血浆）与异种肝细胞之间的相互作用程度。在一个实验模型中，我们给大鼠输注猪肝细胞的培养上清液，并使用蛋白质免疫印迹法和免疫组织学技术证明，大鼠体内产生了针对培养基中极少量猪肝细胞衍生蛋白的抗体。本文讨论了生物人工肝破坏的潜在问题以及由于异种蛋白分泌到患者循环系统中而引发过敏反应的可能性。鉴于肝脏在清除免疫复合物方面具有重要作用，得出的结论是，在考虑（重复）将异种生物人工肝应用于肝功能衰竭患者时应采取预防措施。
