Nishimura M, Uchida S, Mitsunaga S, Yahagi Y, Nakajima K, Tadokoro K, Juji T
Department of Research, Japanese Red Cross, Tokyo, Japan.
Transfus Med. 1997 Jun;7(2):89-94. doi: 10.1046/j.1365-3148.1997.d01-9.x.
We established T cell clones, which were considered to be the possible cause of transfusion-associated graft-versus-host disease (TA-GVHD), from the peripheral blood lymphocytes (PBLs) of two patients. In both cases, several CD4+ cytotoxic T-cell (CTL) clones were established. In case I, the target antigen of the established CD4+ clones was a DRB10403-related antigen serologically typed as HLA DR4, which was one of the patient HLA antigens. In case II, the target of four out of five established CD4+ CTL was a DRB11302-related antigen. One CD4+ CTL clone showed cytotoxicity against cells carrying A2402, B4403, Cw1403 and DPB10401. A monoclonal antibody (mAb) blocking study showed only anti-DP mAb inhibited the cytotoxicity of this clone. Thus, it might be considered that this clone recognizes HLA-DP with its binding peptides derived from either A2402, B4403, Cw1403 or DRB11302. Our findings indicate that CD4+ CTLs may play important roles in the aetiology of TA-GVHD and that the antigens of patients recognized by donor-derived effector cells may not always recognize a single HLA antigen.
我们从两名患者的外周血淋巴细胞（PBL）中建立了T细胞克隆，这些克隆被认为可能是输血相关移植物抗宿主病（TA-GVHD）的病因。在这两个病例中，均建立了多个CD4 + 细胞毒性T细胞（CTL）克隆。在病例I中，所建立的CD4 + 克隆的靶抗原是一种血清学鉴定为HLA DR4的DRB10403相关抗原，它是患者的HLA抗原之一。在病例II中，五个已建立的CD4 + CTL中有四个的靶抗原是DRB11302相关抗原。一个CD4 + CTL克隆对携带A2402、B4403、Cw1403和DPB10401的细胞具有细胞毒性。单克隆抗体（mAb）阻断研究表明，只有抗DP mAb能抑制该克隆的细胞毒性。因此，可以认为该克隆识别带有源自A2402、B4403、Cw1403或DRB11302的结合肽的HLA-DP。我们的研究结果表明，CD4 + CTL可能在TA-GVHD的病因学中起重要作用，并且供体来源的效应细胞识别的患者抗原可能并不总是识别单一的HLA抗原。
