Lauria-Pires L, Santana J M, Tavares F S, Teixeira A R
Laboratório Multidisciplinar de Pesquisa em Doença de Chagas, Faculdade de Ciências da Saúde, Universidade de Brasília, DF.
Rev Soc Bras Med Trop. 1997 May-Jun;30(3):187-92. doi: 10.1590/s0037-86821997000300003.
In this study, we isolated Trypanosoma cruzi from chronic Chagas heart disease and from megaesophagus patients. The parasite stock hSLU239 (heart disease) yielded clones h1 and h2, whereas stock mSEU142 (megaesophagus) yielded clones m1, m2, m3 and m4. The parasite growth kinetics, doubling time and differentiation in axenic liquid medium showed broad behavioral diversity. It was shown that a particular pattern of behavior for a parental stock could not necessarily be assigned for subsequent clones. This study indicates that i) each Chagas disease patient is infected with several T. cruzi populations; ii) clonal lines derived from patient samples may have different biological characteristics from the original isolate; and that iii) additional behavioral and/or molecular markers are required for further characterization of Trypanosoma cruzi stocks and clones derived from Chagas disease patients in order to identify correlations with pathology.
在本研究中，我们从慢性恰加斯心脏病患者和巨食管患者中分离出克氏锥虫。寄生虫株hSLU239（心脏病）产生了克隆h1和h2，而株mSEU142（巨食管）产生了克隆m1、m2、m3和m4。寄生虫在无细胞液体培养基中的生长动力学、倍增时间和分化表现出广泛的行为多样性。结果表明，亲本株的特定行为模式不一定适用于后续克隆。本研究表明：i） 每位恰加斯病患者感染了多个克氏锥虫种群；ii） 从患者样本衍生的克隆系可能具有与原始分离株不同的生物学特征；iii） 需要额外的行为和/或分子标记来进一步表征源自恰加斯病患者的克氏锥虫株和克隆，以便确定与病理学的相关性。
