Higuchi T, Koike K, Sawai N, Koike T
Department of Pediatrics, Shinshu University School of Medicine, Asahi, Matsumoto, Japan.
Exp Hematol. 1997 Jun;25(6):463-70.
We investigated changes in proliferative potential and surface markers during human megakaryocytic differentiation, using megakaryocytic cells grown by thrombopoietin (TPO). Cells grown in response to TPO from CD34+ cord blood cells in a liquid culture system expressed CD41b at a frequency of 92% and CD42b at a frequency of 80% on day 10, whereas cells expressing other lineage markers constituted less than 2.5% of this population. The cultured cells were divided into CD41b-/CD42b-, CD41b+/CD42b-, and CD41b+/CD42b+ cells. Comparison of their respective proliferative potentials showed that CD41b-/CD42b- cells generated megakaryocytic progeny in response to TPO to a lesser extent, but responded to the combination of growth factors (GFs) more intensely than CD41b+/CD42b- cells. Almost all CD41b+/CD42b+ cells failed to undergo cell division. In the culture containing GFs, some CD41b-/CD42b- cells and CD41b+/CD42b- cells gave rise to erythroid as well as megakaryocytic progeny. The potential of these cells to yield erythroid progeny in response to GFs correlated well with their expression of CD34. These results suggest that TPO generates precursors with a potential to differentiate into megakaryocytic and erythroid lineages.
我们使用由血小板生成素（TPO）培养的巨核细胞，研究了人类巨核细胞分化过程中增殖潜能和表面标志物的变化。在液体培养系统中，由CD34 +脐血细胞对TPO产生反应而生长的细胞在第10天时表达CD41b的频率为92%，表达CD42b的频率为80%，而表达其他谱系标志物的细胞占该群体的比例不到2.5%。培养的细胞被分为CD41b - /CD42b -、CD41b + /CD42b -和CD41b + /CD42b +细胞。对它们各自增殖潜能的比较表明，CD41b - /CD42b -细胞对TPO产生巨核细胞后代的程度较小，但对生长因子（GFs）组合的反应比CD41b + /CD42b -细胞更强烈。几乎所有CD41b + /CD42b +细胞都未能进行细胞分裂。在含有GFs的培养物中，一些CD41b - /CD42b -细胞和CD41b + /CD42b -细胞产生了红细胞以及巨核细胞后代。这些细胞对GFs产生红细胞后代的潜能与其CD34的表达密切相关。这些结果表明，TPO产生了具有分化为巨核细胞和红细胞谱系潜能的前体细胞。
