Perez-Oteyza J, Ramos P, Testa N G, Heinrichs B, Lopez-Jimenez J, Garcia-Laraña J, Odriozola J
Department of Hematology, Hospital Ramon y Cajal, Madrid, Spain.
Exp Hematol. 1997 Jun;25(6):516-20.
We evaluated the role of high-dose granulocyte colony stimulating factor (G-CSF) in vitro, in inducing the generation of high-proliferative potential colony forming cells (HPP-CFC), from either mononuclear cells or purified CD34+ cells. Both normal controls and patients undergoing peripheral blood stem cell (PBSC) mobilization and transplantation were studied. In serum-driven agar cultures, G-CSF stimulated the proliferation of HPP-CFC in a dose dependent manner (r = 0.92). The number of HPP-CFC was four-fold greater in mobilized patients than in normal controls. Purified CD34+ cells yielded 11-fold more colonies than mononuclear cells. HPP-CFC from mobilized patients showed replating capacity, giving rise to secondary colonies of more mature appearance. In serum-free cultures, the effect of G-CSF appeared to be mediated by synergistic interaction with stem cell factor. Our results suggest that G-CSF stimulates primitive hematopoietic cells that are detectable in increased amounts in patients receiving mobilization therapy. Therefore, determination of G-CSF induced HPP-CFC could be a useful tool in the evaluation of mobilization strategies.
我们评估了高剂量粒细胞集落刺激因子（G-CSF）在体外诱导单核细胞或纯化的CD34+细胞产生高增殖潜能集落形成细胞（HPP-CFC）中的作用。研究对象包括正常对照组以及接受外周血干细胞（PBSC）动员和移植的患者。在血清驱动的琼脂培养中，G-CSF以剂量依赖性方式刺激HPP-CFC的增殖（r = 0.92）。动员患者的HPP-CFC数量比正常对照组多四倍。纯化的CD34+细胞产生的集落比单核细胞多11倍。动员患者的HPP-CFC表现出再接种能力，可形成外观更成熟的次级集落。在无血清培养中，G-CSF的作用似乎是通过与干细胞因子的协同相互作用介导的。我们的结果表明，G-CSF刺激原始造血细胞，在接受动员治疗的患者中可检测到其数量增加。因此，测定G-CSF诱导的HPP-CFC可能是评估动员策略的有用工具。
