Monsonego J, Valensi P, Zerat L, Clavel C, Birembaut P
Department of Cyto-colposcopic Screening, Alfred Fournier Institute, Paris, France.
Br J Obstet Gynaecol. 1997 Jun;104(6):723-7. doi: 10.1111/j.1471-0528.1997.tb11984.x.
To investigate whether ploidy and oncogenic human papillomavirus types can be correlated with the histological grade of cervical intraepithelial neoplasia (CIN) in the same tissue sections.
Histological data were obtained from 292 dysplastic lesions of the cervix and classified according to the CIN and the Bethesda terminologies. The samples were analysed using Feulgen-stained image analysis cytometry for ploidy and Human papillomaviruses DNA typing by in situ hybridisation, respectively.
Colposcopy Clinic at Alfred Fournier Institute, Paris.
Three hundred and forty women referred for an abnormal cervical smear.
The ploidy data strongly segregate high grade from low grade squamous intraepithelial lesions (aneuploidy 78% versus 21%; P < 0.0001). There was a significant association between aneuploidy and the severity of the lesions (94% for CIN 3, 55% for CIN 2 and 14% for CIN 1; P < 0.0001). Both classifications showed a significant association of histological grade with oncogenic human papillomavirus types (HPV 16-18-33; 20% in low grade and 78% in high grade squamous intraepithelial lesions, 31% and 75% in CIN 1 and CIN 3, respectively; P < 0.0001). The simultaneous effects of these viruses and ploidy demonstrate an association in aneuploid cells between the presence of oncogenic human papillomavirus types and histological grade (76% and 18% in high and low grade squamous intraepithelial lesions, respectively; P < 0.0001). Such an association was not observed in diploid cells (20% in both low and high grade squamous intraepithelial lesions).
High risk human papillomavirus types do not exert and an independent effect on the histological grade of cervical intraepithelial neoplasia.
研究在同一组织切片中，倍体和致癌性人乳头瘤病毒类型是否与宫颈上皮内瘤变（CIN）的组织学分级相关。
获取292例宫颈发育异常病变的组织学数据，并根据CIN和贝塞斯达术语进行分类。分别使用福尔根染色图像分析细胞术分析样本的倍体情况，并通过原位杂交进行人乳头瘤病毒DNA分型。
巴黎阿尔弗雷德·富尼耶研究所阴道镜诊所。
340名因宫颈涂片异常前来就诊的女性。
倍体数据有力地将高级别与低级别鳞状上皮内病变区分开来（非整倍体分别为78%和21%；P<0.0001）。非整倍体与病变严重程度之间存在显著关联（CIN 3为94%，CIN 2为55%，CIN 1为14%；P<0.0001）。两种分类均显示组织学分级与致癌性人乳头瘤病毒类型存在显著关联（HPV 16 - 18 - 33；低级别鳞状上皮内病变中为20%，高级别鳞状上皮内病变中为78%，CIN 1和CIN 3中分别为31%和75%；P<0.0001）。这些病毒和倍体的联合作用表明，在非整倍体细胞中，致癌性人乳头瘤病毒类型的存在与组织学分级之间存在关联（高级别和低级别鳞状上皮内病变中分别为76%和18%；P<0.0001）。在二倍体细胞中未观察到这种关联（低级别和高级别鳞状上皮内病变中均为20%）。
高危型人乳头瘤病毒类型对宫颈上皮内瘤变的组织学分级没有独立影响。
