Aida T, Ishikawa N, Shinkai K
Laboratory Animal Science and Toxicology Laboratories, Sankyo Co., Ltd., Shizuoka, Japan.
J Toxicol Sci. 1997 May;22(2):135-40. doi: 10.2131/jts.22.2_135.
The present experiments were undertaken to clarify the differences in humoral and cellular immune responses to a low-molecular compound, sodium 2,4,6-trinitrobenznnesul fonate dihydrate (TNBS) in guinea-pig strains. Guinea pigs of three different strains, Hartley, Strain 2 and Strain 13, were immunized subcutaneously with TNBS (3 mg/body) 2 or 3 times a week, 9 times in total. Humoral immune responses to TNBS were assessed by passive cutaneous anaphylaxis (PCA) and Arthus reaction, and cellular immune response was assessed by delayed-type hypersensitivity (DTH). Hartley guinea pigs showed high humoral immune responses to TNBS, whereas Strain 2 and 13 guinea pigs showed low responses. Strain 2 guinea pigs displayed high cellular immune response to TNBS, and Strain 13 displayed low cellular immune responses. These results suggest that the pattern of humoral immune response to TNBS does not correlate with that of the cellular immune responses to TNBS for Strain 2 and Hartley guinea pigs.
进行本实验以阐明豚鼠不同品系对低分子化合物2,4,6-三硝基苯磺酸二水合钠（TNBS）的体液免疫和细胞免疫反应的差异。用TNBS（3mg/只）对三种不同品系的豚鼠，即Hartley品系、2号品系和13号品系，每周皮下免疫2或3次，共免疫9次。通过被动皮肤过敏反应（PCA）和阿瑟斯反应评估对TNBS的体液免疫反应，通过迟发型超敏反应（DTH）评估细胞免疫反应。Hartley豚鼠对TNBS表现出高体液免疫反应，而2号和13号品系豚鼠表现出低反应。2号品系豚鼠对TNBS表现出高细胞免疫反应，13号品系表现出低细胞免疫反应。这些结果表明，对于2号品系和Hartley豚鼠，对TNBS的体液免疫反应模式与对TNBS的细胞免疫反应模式不相关。
