一种新型cDNA的分离，其对应的mRNA在生长停滞的汇合大鼠3Y1细胞中积累，而在生长的亚汇合细胞中不积累。

Isolation of a novel cDNA whose corresponding mRNA is accumulated in growth-arrested confluent but not in growing sub-confluent rat 3Y1 cells.

作者信息

Hayashi Y, Kiyono T, Fujita M, Ishibashi M

机构信息

Laboratory of Viral Oncology, Research Institute, Aichi Cancer Center, Chikusa-ku, Nagoya, Japan.

出版信息

Biochim Biophys Acta. 1997 May 30;1352(2):145-50. doi: 10.1016/s0167-4781(97)00051-1.

DOI:10.1016/s0167-4781(97)00051-1

PMID:9199244

Abstract

Four cDNA fragments, whose corresponding mRNAs were accumulated in growth-arrested confluent but not in growing sub-confluent rat 3Y1 cells, were isolated by the mRNA differential display method. Sequencing of the four cDNA fragments indicated that one of them was highly homologous to 3' untranslated DNA region of mouse growth-arrest specific1 cDNA, while the other three (named confluent 3Y1 cell-associated No. 1 (cca1), cca2 and cca3) were novel. Of the three novel cDNAs, we presented some characteristics of cca2 cDNA: the cDNA consisted of 1795 nucleotides with a deduced open reading frame (ORF) encoding a protein of 338 amino acids, which showed a 35% identity with rat type IV 3beta-hydroxysteroid dehydrogenase. The CCA2 protein, with a molecular weight of 38 kDa, was accumulated in 3Y1 cells under growth-arrest conditions.

摘要

通过mRNA差异显示法分离出四个cDNA片段，其相应的mRNA在生长停滞的汇合大鼠3Y1细胞中积累，而在生长的亚汇合大鼠3Y1细胞中未积累。对这四个cDNA片段进行测序表明，其中一个与小鼠生长停滞特异性1 cDNA的3'非翻译DNA区域高度同源，而另外三个（命名为汇合3Y1细胞相关编号1（cca1）、cca2和cca3）是新的。在这三个新的cDNA中，我们展示了cca2 cDNA的一些特征：该cDNA由1795个核苷酸组成，推导的开放阅读框（ORF）编码一个338个氨基酸的蛋白质，该蛋白质与大鼠IV型3β-羟基类固醇脱氢酶具有35%的同一性。分子量为38 kDa的CCA2蛋白在生长停滞条件下在3Y1细胞中积累。