Tanaka S, Yanagisawa N, Tojo H, Kim Y J, Tsujimura T, Kitamura Y, Sawasaki T, Tachi C
Department of Applied Genetics, Institute of Animal Resource Sciences, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Yayoi, Bunkyo-ku, Japan.
Biochim Biophys Acta. 1997 May 30;1352(2):151-5. doi: 10.1016/s0167-4781(97)00055-9.
The complete open reading frame (ORF) of the c-kit cDNA was cloned from a cerebellar cDNA library of the Shiba goat (Capra hircus var Shiba) with the dominant black-eyed white phenotype. The analysis of the deduced amino acid sequence revealed the presence of a single amino acid insertion (alanine) in the kinase insert (KI) region. While the newly found alanine insertion is not correlated with the coat color phenotype of goats, it appears to be characteristic of the c-kit genes in goats and sheep. Although the biological significance of the insert remains to be investigated, its phylogenetically limited distribution will provide us with a useful and interesting tool to analyze the problems of evolution of sheep and goats in bovidae.
从具有显性黑眼白色表型的柴山羊（Capra hircus var Shiba）的小脑cDNA文库中克隆了c-kit cDNA的完整开放阅读框（ORF）。对推导的氨基酸序列分析显示，激酶插入（KI）区域存在单个氨基酸插入（丙氨酸）。虽然新发现的丙氨酸插入与山羊的毛色表型无关，但它似乎是山羊和绵羊c-kit基因的特征。尽管该插入的生物学意义仍有待研究，但其系统发育上有限的分布将为我们提供一个有用且有趣的工具，以分析牛科中绵羊和山羊的进化问题。
