Tanaka K, Handel K, Loewen P C, Takahashi H
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi, Bunkyo-ku, Japan.
Biochim Biophys Acta. 1997 May 30;1352(2):161-6. doi: 10.1016/s0167-4781(97)00044-4.
The rpoS gene of Escherichia coli encodes an alternative sigma factor of RNA polymerase sigma38 (or sigma(s)) that is required for transcription of katE encoding catalase HPII. The transcription start site of the single katE transcript identified by ribonuclease protection has been determined by primer extension analysis to be either 53 or 54 bp (depending on the strain used) upstream of the open reading frame. A series of promoter fragments were constructed and fused to lacZ to confirm the start site location. A - 10 sequence similar to that found in other sigma70- and sigma38-dependent E. coli promoters was identified 8 or 7 bp upstream of the start site but a sigma70-dependent -35 sequence was not evident.
大肠杆菌的rpoS基因编码RNA聚合酶σ38（或σs）的一种替代σ因子，它是编码过氧化氢酶HPII的katE转录所必需的。通过核糖核酸酶保护鉴定的单个katE转录本的转录起始位点，经引物延伸分析确定在开放阅读框上游53或54 bp处（取决于所用菌株）。构建了一系列启动子片段并与lacZ融合，以确认起始位点的位置。在起始位点上游8或7 bp处鉴定出一个与其他依赖σ70和σ38的大肠杆菌启动子中发现的序列相似的-10序列，但未发现依赖σ70的-35序列。
