Dosch R, Gawantka V, Delius H, Blumenstock C, Niehrs C
Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, Heidelberg, Germany.
Development. 1997 Jun;124(12):2325-34. doi: 10.1242/dev.124.12.2325.
The marginal zone is a ring of tissue that gives rise to a characteristic dorsoventral pattern of mesoderm in amphibian embryos. Bmp-4 is thought to play an important role in specifying ventral mesodermal fate. Here we show (1) that different doses of Bmp-4 are sufficient to pattern four distinct mesodermal cell types and to pattern gene expression in the early gastrula marginal zone into three domains, (2) that there is a graded requirement for a Bmp signal in mesodermal patterning, and (3) that Bmp-4 has long-range activity which can become graded in the marginal zone by the antagonizing action of noggin. The results argue that Bmp-4 acts as a morphogen in dorsoventral patterning of mesoderm.
边缘区是一圈组织，在两栖动物胚胎中能产生具有特征性的中胚层背腹模式。Bmp-4被认为在指定腹侧中胚层命运中起重要作用。在此我们表明：（1）不同剂量的Bmp-4足以形成四种不同的中胚层细胞类型，并将原肠胚早期边缘区的基因表达模式化为三个区域；（2）在中胚层模式形成中对Bmp信号存在梯度需求；（3）Bmp-4具有长程活性，其可通过noggin的拮抗作用在边缘区形成梯度。这些结果表明Bmp-4在中胚层的背腹模式形成中充当形态发生素。
