结核分枝杆菌Des蛋白：结核病患者体液免疫反应的一个免疫显性靶点。
Mycobacterium tuberculosis Des protein: an immunodominant target for the humoral response of tuberculous patients.
作者信息
Jackson M, Portnoï D, Catheline D, Dumail L, Rauzier J, Legrand P, Gicquel B
机构信息
Unité de Génétique Mycobactérienne, Institut Pasteur, Paris,
出版信息
Infect Immun. 1997 Jul;65(7):2883-9. doi: 10.1128/iai.65.7.2883-2889.1997.
Abstract
The phoA gene fusion methodology permitted the identification of a new Mycobacterium tuberculosis exported protein, Des. This protein has significant sequence similarities to plant acyl-acyl carrier protein desaturases, which are enzymes involved in general fatty acid biosynthesis as well as in mycolic acid biosynthesis in mycobacteria. As shown by Western blot and enzyme-linked immunosorbent assay experiments, the Des protein is a major B-cell antigen that was recognized by all the tuberculous M. tuberculosis- and M. bovis-infected human patients tested.
摘要
phoA基因融合方法使得一种新的结核分枝杆菌输出蛋白Des得以鉴定。该蛋白与植物酰基-酰基载体蛋白去饱和酶具有显著的序列相似性，这些酶参与一般脂肪酸生物合成以及分枝杆菌中分枝菌酸的生物合成。如蛋白质印迹和酶联免疫吸附测定实验所示，Des蛋白是一种主要的B细胞抗原，在所检测的所有感染结核分枝杆菌和牛分枝杆菌的结核病患者中都能被识别。
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