一种针对97千道尔顿淋球菌血红素结合蛋白的单克隆抗体可抑制淋病奈瑟菌对血红素的利用。
A monoclonal antibody directed against the 97-kilodalton gonococcal hemin-binding protein inhibits hemin utilization by Neisseria gonorrhoeae.
作者信息
Lee B C, Levesque S
机构信息
Department of Microbiology and Infectious Diseases, University of Calgary, Alberta, Canada.
出版信息
Infect Immun. 1997 Jul;65(7):2970-4. doi: 10.1128/iai.65.7.2970-2974.1997.
Abstract
Neisseria gonorrhoeae expresses two hemin-binding proteins (HmBPs) of 97,000 and 44,000 in molecular weight. A murine monoclonal antibody (MAb) produced against the 97-kDa HmBP from N. gonorrhoeae PID543 specifically inhibited in a concentration-dependent manner the ability of hemin to promote growth. The anti-97-kDa HmBP MAb competitively inhibited binding of the 97-kDa HmBP to a hemin-agarose affinity column. In Western immunoblots, the MAb recognized the 97-kDa homologs from a limited survey of clinical gonococcal isolates. These results support the contention that the 97-kDa HmBP is involved in the gonococcal hemin acquisition pathway.
摘要
淋病奈瑟菌表达两种分子量分别为97,000和44,000的血红素结合蛋白（HmBPs）。一种针对淋病奈瑟菌PID543的97-kDa HmBP产生的鼠单克隆抗体（MAb）以浓度依赖性方式特异性抑制血红素促进生长的能力。抗97-kDa HmBP MAb竞争性抑制97-kDa HmBP与血红素-琼脂糖亲和柱的结合。在蛋白质免疫印迹中，该MAb从有限的临床淋病奈瑟菌分离株调查中识别出97-kDa同源物。这些结果支持了97-kDa HmBP参与淋病奈瑟菌血红素获取途径的观点。
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