Packer H L, Lawther H, Armitage J P
Department of Biochemistry, University of Oxford, UK.
FEBS Lett. 1997 Jun 2;409(1):37-40. doi: 10.1016/s0014-5793(97)00473-0.
The rotation rate of the unidirectional stop/start motor of Rhodobacter sphaeroides was investigated using computerised motion analysis of tethered cells. The R. sphaeroides motor was found to have a variable rotation rate compared to the virtually constant-speed motor of wild-type and CheR mutant (smooth swimming) Escherichia coli. In addition, the dynamics of the R. sphaeroides motor during stopping was analysed with no consistent correlation behaviour. The motor could go from full rotation to stop, or stop to full rotation within one video frame, i.e. 0.02 s, but it could also slow down into a stop or restart slowly, taking up to 0.25 s. The R. sphaeroides motor under chemokinetic stimulation was also analysed and was found to show increased torque generation and reduced variation in rotation rate.
利用对系留细胞的计算机化运动分析,研究了球形红细菌单向停止/启动马达的转速。与野生型和CheR突变体(平滑游动)大肠杆菌的几乎恒速马达相比,发现球形红细菌的马达具有可变的转速。此外,分析了球形红细菌马达在停止过程中的动力学,未发现一致的相关行为。该马达可以在一个视频帧(即0.02秒)内从全速旋转变为停止,或从停止变为全速旋转,但也可能减速至停止或缓慢重启,耗时可达0.25秒。还分析了化学动力学刺激下的球形红细菌马达,发现其扭矩产生增加,转速变化减小。