Aoki E, Takeuchi I K
Department of Embryology, Aichi Human Service Center, Japan.
J Histochem Cytochem. 1997 Jun;45(6):875-81. doi: 10.1177/002215549704500612.
Using antibodies highly specific for L-arginine and L-citrulline, we localized these amino acids in rat kidney with immunohistochemical methods. Highest levels of arginine immunoreactivity were observed in epithelial cells of proximal tubules in the outer stripe of the outer medulla and the collecting ducts in the cortex. Staining intensity of proximal convoluted tubules in the outer stripe decreased from the inner side to the outer side. In the inner medulla, collecting ducts were labeled with moderate intensity. Staining within the cortex was apparent only with collecting ducts. Citrulline immunoreactivity was localized in the epithelial cells of collecting ducts both in the cortex and medulla. Immunoreactivity was also found in glomerular podocytes and in the epithelial cells of proximal convoluted tubules in the outer medulla. These localizations were different from those of other amino acids previously reported. The precise cellular distribution of arginine and citrulline in rat kidney was determined for the first time by an immunohistochemical method in the present study.
我们使用对L-精氨酸和L-瓜氨酸具有高度特异性的抗体，通过免疫组织化学方法在大鼠肾脏中定位这些氨基酸。在外髓质外带的近端小管上皮细胞和皮质的集合管中观察到最高水平的精氨酸免疫反应性。外带近端曲管的染色强度从内侧到外侧逐渐降低。在内髓质中，集合管被中等强度标记。皮质内仅集合管有明显染色。瓜氨酸免疫反应性定位于皮质和髓质的集合管上皮细胞中。在肾小球足细胞和外髓质近端曲管的上皮细胞中也发现了免疫反应性。这些定位与先前报道的其他氨基酸的定位不同。本研究首次通过免疫组织化学方法确定了大鼠肾脏中精氨酸和瓜氨酸的确切细胞分布。
