Smider V, Chu G
Department of Medicine, Stanford University Medical Center, CA 94305, USA.
Semin Immunol. 1997 Jun;9(3):189-97. doi: 10.1006/smim.1997.0070.
V(D)J recombination consists of a DNA cleavage reaction catalysed by RAG1 and RAG2, followed by an end-joining reaction that utilizes the cell's double-strand break repair machinery. Genes essential for the end-joining reaction include: XRCC4 encoding a protein of unknown enzymatic function; XRCC5 and XRCC6 encoding 86 and 70 kDa subunits of the Ku autoantigen, a DNA end-binding protein that is also the regulatory subunit of DNA-dependent protein kinase (DNA-PK); and XRCC7 encoding the catalytic subunit (DNA-PKcs) of DNA-PK. Recent progress in understanding the cleavage reaction, coupled with what was previously known about Ku, DNA-PK, and double-strand break repair, provide the foundation for a working model of how V(D)J recombination might be catalysed.
V(D)J重组包括由RAG1和RAG2催化的DNA切割反应，随后是利用细胞双链断裂修复机制的末端连接反应。末端连接反应所必需的基因包括：编码酶功能未知的蛋白质的XRCC4；编码Ku自身抗原86 kDa和70 kDa亚基的XRCC5和XRCC6，Ku自身抗原是一种DNA末端结合蛋白，也是DNA依赖性蛋白激酶（DNA-PK）的调节亚基；以及编码DNA-PK催化亚基（DNA-PKcs）的XRCC7。在理解切割反应方面的最新进展，结合先前对Ku、DNA-PK和双链断裂修复的了解，为V(D)J重组如何被催化的工作模型提供了基础。
