High-performance liquid chromatographic determination of cisplatin as platinum(II) in a pharmaceutical preparation and blood samples of cancer patients.

An high-performance liquid chromatographic (HPLC) method has been developed for the determination of cisplatin, based on precolumn derivatization of platinum(II) with bis(salicylaldehyde)tetramethylethylenediimine, extraction in chloroform and elution from a 3 microm Hypersil ODS column with methanol-acetonitrile-water as mobile phase and detection at 254 nm. Copper(II), iron(II), nickel(II), palladium(II), dioxouranium(IV) separated completely and did not affect the determination of platinum(II). The method was applied for the determination of cisplatin as platinum(II) in a pharmaceutical preparation and in blood samples of cancer patients after infusion of cisplatin.