高效液相色谱法测定药物制剂和癌症患者血样中顺铂（以铂（II）形式）的含量。
High-performance liquid chromatographic determination of cisplatin as platinum(II) in a pharmaceutical preparation and blood samples of cancer patients.
作者信息
Khuhawar M Y, Lanjwani S N, Memon S A
机构信息
Institute of Chemistry, University of Sindh, Jamshoro, Pakistan.
出版信息
J Chromatogr B Biomed Sci Appl. 1997 May 23;693(1):175-9. doi: 10.1016/s0378-4347(97)00038-8.
An high-performance liquid chromatographic (HPLC) method has been developed for the determination of cisplatin, based on precolumn derivatization of platinum(II) with bis(salicylaldehyde)tetramethylethylenediimine, extraction in chloroform and elution from a 3 microm Hypersil ODS column with methanol-acetonitrile-water as mobile phase and detection at 254 nm. Copper(II), iron(II), nickel(II), palladium(II), dioxouranium(IV) separated completely and did not affect the determination of platinum(II). The method was applied for the determination of cisplatin as platinum(II) in a pharmaceutical preparation and in blood samples of cancer patients after infusion of cisplatin.
已开发出一种高效液相色谱（HPLC）法用于测定顺铂，该方法基于铂（II）与双（水杨醛）四甲基乙二胺的柱前衍生化、在氯仿中的萃取以及以甲醇 - 乙腈 - 水为流动相从3微米的Hypersil ODS柱上洗脱，并在254纳米处进行检测。铜（II）、铁（II）、镍（II）、钯（II）、二氧化铀（IV）能完全分离，且不影响铂（II）的测定。该方法用于测定药物制剂中的顺铂以及癌症患者输注顺铂后血样中的铂（II）。
Zotero 插件