Carlucci G, Mazzeo P
Dipartimento di Chimica, Ingegneria Chimica e Materiali, Università dell'Aquila, Coppito (L'Aquila), Italy.
J Chromatogr B Biomed Sci Appl. 1997 May 23;693(1):245-8. doi: 10.1016/s0378-4347(97)00045-5.
A simple, specific and sensitive high-performance liquid chromatographic method has been developed and validated for the determination of finasteride in human plasma. A solid-phase extraction procedure was used to isolate finasteride from the biological matrix before quantitative analysis. The analyte was separated on a Symmetry reversed-phase column using acetonitrile-0.04 M orthophosphoric acid (pH 4.0) as mobile phase and quantified by measuring its UV absorbance at 215 nm. The limit of detection for the analyte was 0.005 microg/ml. 4-Androstene-3,17-dione was used as internal standard. The calibration graph of the method was linear from 0.01 to 3.0 microg/ml of finasteride in human plasma, and the coefficient of variation less than 4.5%. This HPLC procedure is simple, precise and accurate for the determination of finasteride in human plasma.
已开发并验证了一种简单、特异且灵敏的高效液相色谱法，用于测定人血浆中的非那雄胺。在定量分析前，采用固相萃取程序从生物基质中分离非那雄胺。分析物在Symmetry反相柱上进行分离，以乙腈-0.04 M正磷酸（pH 4.0）为流动相，并通过测量其在215 nm处的紫外吸光度进行定量。分析物的检测限为0.005微克/毫升。4-雄烯-3,17-二酮用作内标。该方法的校准曲线在人血浆中非那雄胺浓度为0.01至3.0微克/毫升范围内呈线性，变异系数小于4.5%。这种高效液相色谱法用于测定人血浆中的非那雄胺简单、精确且准确。
