Palea S, Angel I
Synthélabo Recherche, Department of Internal Medicine, Rueil-Malmaison, France.
Life Sci. 1997;61(1):PL21-6. doi: 10.1016/s0024-3205(97)00363-9.
Contractile responses induced by carbachol on the detrusor muscle and by noradrenaline on the isolated urethra were compared between ovariectomized rats pretreated with estradiol (50 microg/animal s.c. twice daily for five days), untreated ovariectomized rats and intact animals. In the detrusor muscle, contractions induced by 30 microM carbachol, when normalized with respect to KCl 100 mM-induced contraction, were similar for the three groups. Furthermore, contractions induced by 100 microM noradrenaline in the isolated urethra were not significatively different between groups. However, the pD2 value for noradrenaline was greater in urethral tissue from ovariectomized rats compared with ovariectomized -estrogen treated and control rats. A similar result was found for pD2 values for carbachol-induced contractions on the detrusor muscle. These results suggest that ovariectomy increases the sensitivity of the urinary bladder and urethra to the contractile effects of carbachol and noradrenaline, respectively and that this effect is reversed by in vivo estrogen pretreatment.
比较了用雌二醇预处理（50微克/只动物，皮下注射，每日两次，共五天）的去卵巢大鼠、未处理的去卵巢大鼠和完整动物中，卡巴胆碱对逼尿肌的收缩反应以及去甲肾上腺素对离体尿道的收缩反应。在逼尿肌中，当以100 mM氯化钾诱导的收缩进行标准化时，三组中由30 microM卡巴胆碱诱导的收缩相似。此外，三组之间，100 microM去甲肾上腺素在离体尿道中诱导的收缩没有显著差异。然而，与去卵巢-雌激素处理大鼠和对照大鼠相比，去卵巢大鼠尿道组织中去甲肾上腺素的pD2值更大。在卡巴胆碱诱导的逼尿肌收缩的pD2值方面也发现了类似结果。这些结果表明，去卵巢分别增加了膀胱和尿道对卡巴胆碱和去甲肾上腺素收缩作用的敏感性，并且这种作用可通过体内雌激素预处理逆转。
