Wad N, Guenat C, Krämer G
Swiss Epilepsy Center, Zürich, Switzerland.
Ther Drug Monit. 1997 Jun;19(3):314-7. doi: 10.1097/00007691-199706000-00012.
In this article, the authors discuss 9-hydroxymethyl-10-carbamoyl acridan (9-OH-CBZ), another metabolite of carbamazepine (CBZ) found in serum. The retention time of unconjugated 9-OH-CBZ should be known when using a chromatographic method, because it appears in concentrations varying from one eighth to one third of the CBZ-10,11-epoxide (CBZ-E) concentration and may therefore cause analytical interactions. Liquid chromatography/electrospray mass spectrometry ((LC/ES-MS) in a serum extract identified and confirmed 9-OH-CBZ. The amount of 9-OH-CBZ present as conjugate in serum was between 42% and 65%. The correlation factor values (r) between serum concentrations of 9-OH-CBZ and 10,11-dihydro-10,11-trans-dihydroxy-CBZ (trans-CBZ-diol), CBZ-E, and CBZ in 100 serum samples were 0.77, 0.80, and 0.53, respectively. The origin of 9-OH-CBZ is discussed.
在本文中，作者讨论了9-羟甲基-10-氨基甲酰吖啶（9-OH-CBZ），它是在血清中发现的卡马西平（CBZ）的另一种代谢产物。使用色谱法时应了解未结合的9-OH-CBZ的保留时间，因为它的浓度在CBZ-10,11-环氧化物（CBZ-E）浓度的八分之一到三分之一之间变化，因此可能会引起分析干扰。液相色谱/电喷雾质谱法（LC/ES-MS）在血清提取物中鉴定并确认了9-OH-CBZ。血清中以结合物形式存在的9-OH-CBZ含量在42%至65%之间。100份血清样本中9-OH-CBZ与10,11-二氢-10,11-反式二羟基-CBZ（反式-CBZ-二醇）、CBZ-E和CBZ血清浓度之间的相关系数值（r）分别为0.77、0.80和0.53。本文还讨论了9-OH-CBZ的来源。
