Mashima J, Nakada K, Miyazaki J I, Hirabayashi T
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
Zoolog Sci. 1997 Feb;14(1):109-14. doi: 10.2108/zsj.14.109.
Cells prepared from chicken skeletal muscles of early developmental stages were cultured to study their troponin T isoform expression, using antisera specific to fast- and slow-muscle-type isoforms, and compared with the cells from later stages described in the previous study (Mashima at al., 1996). We found that cultured myogenic cells from chickens and chick embryos could be classified, as in the previous study, into two types, fast type and fast/slow type in which fast- and slow-muscle-type isoforms were coexpressed. Ratios of these two types of muscle cells varied depending on their origins and developmental stages, and fast/slow type cells were in the majority at early stages. Since two distinct populations of cells committed to myogenic cell lineages were supposed to give rise to the two types of myotubes, we investigated the intrinsic stability of troponin T expression of the cultured myogenic cells using the serial subcloning method. The results of clonal analysis suggested that the expression pattern of troponin T isoform in cultured muscle cells is stable and that myogenic cell lineages play an important role in giving rise to different muscle types.
从早期发育阶段的鸡骨骼肌制备细胞，使用针对快肌型和慢肌型同工型的抗血清培养，以研究它们的肌钙蛋白T同工型表达，并与先前研究（Mashima等人，1996年）中描述的后期阶段的细胞进行比较。我们发现，与先前的研究一样，来自鸡和鸡胚胎的培养成肌细胞可分为两种类型，即快肌型和快/慢型，其中快肌型和慢肌型同工型共表达。这两种类型的肌肉细胞的比例因起源和发育阶段而异，并且快/慢型细胞在早期占多数。由于假定两个不同的成肌细胞谱系群体产生两种类型的肌管，我们使用连续亚克隆方法研究了培养的成肌细胞中肌钙蛋白T表达的内在稳定性。克隆分析结果表明，培养的肌肉细胞中肌钙蛋白T同工型的表达模式是稳定的，并且成肌细胞谱系在产生不同肌肉类型中起重要作用。
