Garcia-Isidoro M, Tabernero M D, Najera M L, Lopez-Berges M C, Martinez A, Durán A, Garcia J L, Hernandez J M, Garcia Marcos M A, San Miguel J F, Orfao A
Servicio General de Citometria, Hospital Universitario de Salamanca, Spain.
Ann Hematol. 1997 May;74(5):209-14. doi: 10.1007/s002770050286.
In the present study we have used FISH to analyze the incidence of trisomy 8 in acute leukemias following either a primary myeloproliferative disorder (MPD) or a myelodysplastic syndrome (MDS) and correlated it with both the immunophenotype and the cell-cycle distribution of the leukemic blast cells. Six of the 21 (28%) acute leukemias studied displayed trisomy 8 by FISH. The number of trisomic cells in these cases ranged from 20 to 84%, with a mean of 46 +/- 24%. Trisomy 8 was associated with a homogeneous population of leukemic cells, phenotypically characterized by CD34+/HLADR+/CD13+/CD33+/CD11b-/ CD15-/CD14-. No significant differences were observed on the proliferative rate of cases with trisomy 8, as compared with blast cells from the remaining patients. Overall, our findings suggest that in acute leukemias secondary to MPD or MDS, trisomy 8 is associated with a blockade of myeloid maturation at an early step of the differentiation process.
在本研究中，我们运用荧光原位杂交技术（FISH）分析了原发性骨髓增殖性疾病（MPD）或骨髓增生异常综合征（MDS）后急性白血病中8号染色体三体的发生率，并将其与白血病原始细胞的免疫表型和细胞周期分布相关联。在研究的21例急性白血病中，有6例（28%）通过FISH显示存在8号染色体三体。这些病例中三体细胞的数量在20%至84%之间，平均为46±24%。8号染色体三体与白血病细胞的均一群体相关，其表型特征为CD34+/HLADR+/CD13+/CD33+/CD11b-/CD15-/CD14-。与其余患者的原始细胞相比，8号染色体三体病例的增殖率未观察到显著差异。总体而言，我们的研究结果表明，在MPD或MDS继发的急性白血病中，8号染色体三体与分化过程早期的髓系成熟阻滞有关。
