Prevost-Blondel A, Lengagne R, Letourneur F, Pannetier C, Gomard E, Guillet J G
Institut National de la Santé et de la Recharche Médicale, Unit 445, Paris, France.
Virology. 1997 Jun 23;233(1):93-104. doi: 10.1006/viro.1997.8604.
Recent studies have demonstrated biased usage of TCR V beta 17 and a high degree of diversity in J beta usage within the influenza virus matrix epitope (M.58-66)-specific CTL response. In contrast, in the course of a study on the cellular response to influenza A virus, we found preferential usage of V beta 17-J beta 2.2 rearrangement in an individual with an unexpectedly high number of CTL precursors (CTLp). We took advantage of such situation to study the longitudinal repertoire of the CD8+ T cell precursors. By limiting dilution analysis combined with the use of a clonotypic primer corresponding to the CDR3 region of this matrix-specific TCR V beta chain, the influenza-specific CTLp were shown to be stable for a period of 6 years. Overall, our results show that virus-specific CTLp can be directly monitored in vivo by molecular fingerprinting without in vitro restimulation. These findings might be extremely important for evaluation of the specific immune response to a given human pathogen.
最近的研究表明，在流感病毒基质表位（M.58 - 66）特异性CTL反应中，TCR Vβ17存在偏向性使用，且Jβ使用具有高度多样性。相比之下，在一项关于甲型流感病毒细胞反应的研究过程中，我们发现一名具有意外高数量CTL前体（CTLp）的个体优先使用Vβ17 - Jβ2.2重排。我们利用这种情况研究了CD8 + T细胞前体的纵向库。通过有限稀释分析并结合使用与该基质特异性TCR Vβ链的CDR3区域相对应的克隆型引物，流感特异性CTLp在6年的时间内表现出稳定性。总体而言，我们的结果表明，无需体外再刺激，通过分子指纹图谱可在体内直接监测病毒特异性CTLp。这些发现对于评估针对特定人类病原体的特异性免疫反应可能极为重要。
