Cruz M, Isibasi A, de Alvarez-Buylla E R, Alvarez-Buylla R
CUIB, Universidad de Colima, Col.
Acta Physiol Pharmacol Ther Latinoam. 1996;46(3):169-76.
The response of hypophysectomized (HYPOX) and sham-operated (S-HYPOX) female and male Wistar young rats (8 weeks old) to antigenic stimulation was compared. Humoral antigenic responses against hemocyanin were measured by ELISA. [3H]thymidine incorporation into cultured spleen cells was used to determine proliferative response to concanavalin A (ConA) or antigenic stimulation. Anti-hemocyanin serum titers in the HYPOX animals was about half of that observed in control S-HYPOX rats. Similarly, the cellular proliferative response was significantly decreased in HYPOX animals when compared to S-HYPOX rats; the blastogenic response to hemocyanin in UC rats (which did not receive the antigen injection) was close to zero. S-HYPOX control rats responded to direct ConA stimulation as UC controls. Body weight and the weight of pituitary target organs (adrenal, thyroid, ovary and testes) was about 1/4 of that of controls. Hypophysectomy also resulted in a striking reduction in spleen weight. These results indicate that the pituitary gland is involved in cellular and humoral immune regulation in young rats.
比较了垂体切除（HYPOX）和假手术（S - HYPOX）的8周龄雌性和雄性Wistar幼鼠对抗原刺激的反应。通过ELISA测定针对血蓝蛋白的体液抗原反应。利用[³H]胸苷掺入培养的脾细胞来确定对刀豆球蛋白A（ConA）或抗原刺激的增殖反应。HYPOX动物的抗血蓝蛋白血清滴度约为对照S - HYPOX大鼠的一半。同样，与S - HYPOX大鼠相比，HYPOX动物的细胞增殖反应显著降低；未接受抗原注射的UC大鼠对血蓝蛋白的母细胞形成反应接近零。S - HYPOX对照大鼠对直接ConA刺激的反应与UC对照相同。体重以及垂体靶器官（肾上腺、甲状腺、卵巢和睾丸）的重量约为对照的1/4。垂体切除还导致脾脏重量显著减轻。这些结果表明垂体参与幼鼠的细胞和体液免疫调节。
