Ueda Y, Yokoyama H, Ohya-Nishiguchi H, Kamada H
Institute for Life Support Technology, Yamagata Technopolis Foundation, Japan.
Magn Reson Imaging. 1997;15(3):355-60. doi: 10.1016/s0730-725x(96)00141-5.
We report here our investigation of the spatial distribution of free radicals using an electron spin resonance (ESR)-imaging system combined with an in vivo brain microdialysis method, which was performed in the resonator of the ESR-imaging system. A nonmagnetic cannula, newly developed in this study, was used for the perfusion of the exogenous free radicals agent. A nitroxide, 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl (carbamoyl PROYXL), was used as the imaging agent in saline solution at a concentration of 0.3 M, which was perfused into the right caudate putamen of the rat at 2 microliters/min by a microinfusion pump. Two-dimensional ESR projection of the Z-X plane, which was clearly distinguished (about phi 10 mm) from the nonperfused brain area, was obtained 6 h after the beginning of perfusion of carbamoyl PROXYL. The present method is considered to be a useful tool to introduce stable free radicals into a specific area of the brain.
我们在此报告，我们使用电子自旋共振（ESR）成像系统结合体内脑微透析方法，对自由基的空间分布进行了研究，该研究在ESR成像系统的谐振器中进行。本研究新开发的一种非磁性套管用于灌注外源性自由基剂。一种氮氧化物，3-氨基甲酰基-2,2,5,5-四甲基吡咯烷-1-氧基（氨基甲酰基PROYXL），以0.3 M的浓度溶解在盐溶液中用作成像剂，通过微量注射泵以2微升/分钟的速度灌注到大鼠的右侧尾状壳核中。在开始灌注氨基甲酰基PROXYL后6小时，获得了Z-X平面的二维ESR投影，该投影与未灌注的脑区明显区分开（约φ10毫米）。本方法被认为是将稳定自由基引入脑特定区域的一种有用工具。
