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将大肠杆菌外膜蛋白FhuA重组到脂质体中。噬菌体T5与Fhua的结合触发DNA向蛋白脂质体的转移。

Reconstitution of FhuA, an Escherichia coli outer membrane protein, into liposomes. Binding of phage T5 to Fhua triggers the transfer of DNA into the proteoliposomes.

作者信息

Plançon L, Chami M, Letellier L

机构信息

Laboratoire des Biomembranes, URA CNRS 1116, Université Paris-Sud, Bât 430, F-91405 Orsay Cedex, France.

出版信息

J Biol Chem. 1997 Jul 4;272(27):16868-72. doi: 10.1074/jbc.272.27.16868.

DOI:10.1074/jbc.272.27.16868
PMID:9201994
Abstract

The Escherichia coli outer membrane protein FhuA catalyzes the transport of ferrichrome and is the receptor of bacteriophage T5. Purified FhuA was reconstituted into liposomes. The size of the proteoliposomes and the distribution of the proteins in the vesicles were determined by freeze fracture electron microscopy. Unilamellar vesicles with a diameter larger than 200 nm were observed frequently. FhuA was symetrically oriented in the proteoliposomes. Reconstituted FhuA was functional as binding of phage T5 induced the release of phage DNA and its transfer inside the vesicles.

摘要

大肠杆菌外膜蛋白FhuA催化高铁色素的转运，是噬菌体T5的受体。将纯化的FhuA重组到脂质体中。通过冷冻断裂电子显微镜确定了蛋白脂质体的大小和蛋白质在囊泡中的分布。经常观察到直径大于200 nm的单层囊泡。FhuA在蛋白脂质体中呈对称取向。重组的FhuA具有功能，因为噬菌体T5的结合诱导了噬菌体DNA的释放及其在囊泡内的转移。

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