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一种新型甘丙肽样肽内源性产生的可能证据。

Possible evidence for endogenous production of a novel galanin-like peptide.

作者信息

Wang Z L, Kulkarni R N, Wang R M, Smith D M, Ghatei M A, Byfield P G, Bennet W M, Bloom S R

机构信息

Francis Fraser Labs, Division of Endocrinology and Metabolic Medicine and Haemostasis Research Unit, Hammersmith Hospital, Royal Postgraduate Medical School, London W12 0NN, United Kingdom.

出版信息

J Clin Invest. 1997 Jul 1;100(1):189-96. doi: 10.1172/JCI119512.

DOI:10.1172/JCI119512
PMID:9202071
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC508179/
Abstract

Galanin mRNA and peptide are not detectable in normal islets. We studied the effect of galanin antagonists on insulin secretion in the rat beta cell line, RIN5AH, and in perifused rat islets. In RIN cell membranes galanin and its antagonists showed high affinity for 125I-galanin binding sites [Kd: (galanin) 0.03+/-0.01; Ki for galanin antagonists: (C7) 0.12+/- 0.02, (M35) 0.21+/-0.04, and (M40) 0.22+/-0.03 nM, mean+/- SEM, n = 4]. Galanin (1 microM) inhibited glucose-induced insulin release in islets (control 21.2+/-1.5 vs. galanin 4.5+/-0.2 fmol/islet per min, P < 0.001, n = 6) and RIN5AH cells (control 0.26+/-0.01 vs. galanin 0.15+/-0.02 pmol/10(6) cells per h, P < 0.001, n = 9). In RIN5AH cells, all antagonists blocked the inhibitory effects of galanin and stimulated insulin release in the absence of galanin. C7 and M40 (1 microM) alone significantly stimulated glucose-induced insulin secretion. Purified porcine galanin antibody (GAb) enhanced glucose-induced insulin release from islets (control 100+/- 16.3% vs. GAb 806.1+/-10.4%, P < 0.001, n = 6), and RIN5AH cells (control 100+/-9.6% vs. GAb 149+/-6.8%, P < 0. 01, n = 6). Western blotting of dexamethasone-treated islet extracts using GAb showed a specific band of similar molecular weight to porcine galanin not detected using a rat specific galanin antibody. One possible explanation for these results is the presence of an endogenous galanin-like peptide.

摘要

在正常胰岛中检测不到甘丙肽信使核糖核酸和肽。我们研究了甘丙肽拮抗剂对大鼠β细胞系RIN5AH和灌流大鼠胰岛胰岛素分泌的影响。在RIN细胞膜中，甘丙肽及其拮抗剂对125I-甘丙肽结合位点显示出高亲和力[解离常数：（甘丙肽）0.03±0.01；甘丙肽拮抗剂的抑制常数：（C7）0.12±0.02，（M35）0.21±0.04，以及（M40）0.22±0.03纳摩尔，均值±标准误，n = 4]。甘丙肽（1微摩尔）抑制胰岛中葡萄糖诱导的胰岛素释放（对照组21.2±1.5对甘丙肽组4.5±0.2飞摩尔/胰岛每分钟，P < 0.001，n = 6）以及RIN5AH细胞中葡萄糖诱导的胰岛素释放（对照组0.26±0.01对甘丙肽组0.15±0.02皮摩尔/10⁶细胞每小时，P < 0.001，n = 9）。在RIN5AH细胞中，所有拮抗剂均阻断了甘丙肽的抑制作用，并在无甘丙肽时刺激胰岛素释放。单独使用C7和M40（1微摩尔）可显著刺激葡萄糖诱导的胰岛素分泌。纯化的猪甘丙肽抗体（GAb）增强了胰岛中葡萄糖诱导的胰岛素释放（对照组100±16.3%对GAb组806.1±10.4%，P < 0.001，n = 6）以及RIN5AH细胞中葡萄糖诱导的胰岛素释放（对照组100±9.6%对GAb组149±6.8%，P < 0.01，n = 6）。使用GAb对经地塞米松处理的胰岛提取物进行蛋白质印迹分析显示，存在一条与猪甘丙肽分子量相似的特异性条带，而使用大鼠特异性甘丙肽抗体则未检测到该条带。这些结果的一种可能解释是存在内源性甘丙肽样肽。

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本文引用的文献

1
Expression of adrenomedullin and its receptor during embryogenesis suggests autocrine or paracrine modes of action.
Endocrinology. 1997 Jan;138(1):440-51. doi: 10.1210/endo.138.1.4881.
2
Dexamethasone-induced neuropeptide Y expression in rat islet endocrine cells. Rapid reversibility and partial prevention by insulin.地塞米松诱导大鼠胰岛内分泌细胞中神经肽Y的表达。胰岛素可使其快速逆转并部分预防。
Diabetes. 1996 Oct;45(10):1306-16. doi: 10.2337/diab.45.10.1306.
3
Binding and agonist/antagonist actions of M35, galanin(1-13)-bradykinin(2-9)amide chimeric peptide, in Rin m 5F insulinoma cells.M35（甘丙肽(1-13)-缓激肽(2-9)酰胺嵌合肽）在Rin m 5F胰岛素瘤细胞中的结合及激动剂/拮抗剂作用
Regul Pept. 1995 Nov 10;59(3):341-8. doi: 10.1016/0167-0115(95)00089-t.
4
Influence of islet amyloid polypeptide and the 8-37 fragment of islet amyloid polypeptide on insulin release from perifused rat islets.胰岛淀粉样多肽及胰岛淀粉样多肽8-37片段对经灌流的大鼠胰岛胰岛素释放的影响。
Diabetes. 1993 Feb;42(2):330-5. doi: 10.2337/diab.42.2.330.
5
Evidence of a paracrine role of neuropeptide-Y in the regulation of insulin release from pancreatic islets of normal and dexamethasone-treated rats.神经肽Y在正常及地塞米松处理大鼠胰岛胰岛素释放调节中旁分泌作用的证据。
Endocrinology. 1994 Jul;135(1):200-6. doi: 10.1210/endo.135.1.8013354.
6
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